Keeler R F, Johnson A E, Chase R L
Cornell Vet. 1986 Apr;76(2):115-27.
Cattle had severe signs of toxicosis when gavaged dried ground Thermopsis montana (false lupine, poison bean, mountain thermopsis) at doses of 0.6-2.8 g/kg/day in a water suspension. Signs included depression, anorexia, swollen eye lids, arched back, tucked abdomen, rough hair coat, and in extremis a prolonged recumbency lasting up to 9 days. Plant potency varied among collections. Total alkaloid doses in collections eliciting severe signs varied from 1.1-11.3 mg/kg/day. There were 5 major alkaloids in each collection that varied in concentration among the collections perhaps accounting for variation in severity of signs elicited. Four of the alkaloids were identified by GC retention time, MS fragmentation patterns and OR analysis as N-methylcytisine, cytisine, (-)-thermopsine, and (-)-anagyrine. Measurements showed a very marked increase of 10X-20X in levels of certain serum enzymes--SGOT, CPK, and LDH that persisted during the period of maximum clinical signs.
当以0.6 - 2.8克/千克/天的剂量，用悬浮于水中的干燥蒙大拿野决明（假羽扇豆、毒豆、山地野决明）灌喂牛时，牛出现了严重的中毒症状。症状包括精神沉郁、厌食、眼睑肿胀、背部拱起、腹部蜷缩、被毛粗糙，极度情况下会出现长达9天的长时间躺卧。不同采集批次的植物效力有所不同。引发严重症状的采集批次中，总生物碱剂量为1.1 - 11.3毫克/千克/天。每个采集批次中有5种主要生物碱，各批次间其浓度有所差异，这可能是导致所引发症状严重程度不同的原因。通过气相色谱保留时间、质谱碎片模式和旋光分析，鉴定出其中4种生物碱为N - 甲基金雀花碱、金雀花碱、（-）-野决明碱和（-）-臭豆碱。检测显示，某些血清酶——谷草转氨酶、肌酸磷酸激酶和乳酸脱氢酶的水平显著升高了10至20倍，且在临床症状最严重的时期一直持续。
