Edelstein Inna Alexandrovna, Guschin Alexandr Evgenjevich, Romanov Andrew Vyacheslavovich, Negasheva Ekaterina Sergeevna, Kozlov Roman Sergeevich
Institute of Antimicrobial Chemotherapy, Smolensk State Medical University of the Ministry of Health of the Russian Federation, 214019 Smolensk, Russia.
Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology, 125008 Moscow, Russia.
Pathogens. 2023 Mar 22;12(3):496. doi: 10.3390/pathogens12030496.
Macrolide (MLR) and fluoroquinolone (FQR) resistance in (MG) has recently become a major problem worldwide. The available data on the prevalence of MLR and FQR in MG in Russia are limited. In this study, we aimed to evaluate the prevalence and pattern of mutations in 213 MG-positive urogenital swabs from patients in Moscow between March 2021 and March 2022. MLR- and FQR-associated mutations were searched in 23S rRNA as well as in the parC and gyrA genes using Sanger sequencing. The prevalence of MLR was 55/213 (26%), with A2059G and A2058G substitutions being the two most common variants (36/55, 65%, and 19/55, 35%, respectively). FQR detection showed 17% (37/213); two major variants were D84N (20/37, 54%) and S80I (12/37, 32.4%) and three minor variants were S80N (3/37, 8.1%), D84G (1/37, 2.7%), and D84Y (1/37, 2.7%). Fifteen of the fifty-five MLR cases (27%) simultaneously harbored FQR. This study revealed the high frequency of MLR and FQR. We conclude that the improvement of patient examination algorithms and therapeutic approaches should be combined with the routine monitoring of antibiotic resistance based on the sensitivity profiles presented. Such a complex approach will be essential for restraining the development of treatment resistance in MG.
支原体（MG）对大环内酯类（MLR）和氟喹诺酮类（FQR）的耐药性近来已成为全球范围内的一个主要问题。俄罗斯关于MG中MLR和FQR流行情况的现有数据有限。在本研究中，我们旨在评估2021年3月至2022年3月期间来自莫斯科患者的213份MG阳性泌尿生殖系统拭子中突变的流行情况和模式。使用桑格测序法在23S rRNA以及parC和gyrA基因中搜索与MLR和FQR相关的突变。MLR的流行率为55/213（26%），A2059G和A2058G替换是两个最常见的变体（分别为36/55，65%，以及19/55，35%）。FQR检测显示为17%（37/213）；两个主要变体为D84N（20/37，54%）和S80I（12/37，32.4%），三个次要变体为S80N（3/37，8.1%）、D84G（1/37，2.7%）和D84Y（1/37，2.7%）。55例MLR病例中有15例（27%）同时携带FQR。本研究揭示了MLR和FQR的高频率。我们得出结论，患者检查算法和治疗方法的改进应与基于所呈现的敏感性谱对抗生素耐药性的常规监测相结合。这种综合方法对于抑制MG治疗耐药性的发展至关重要。
