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蛋白质含量和SDS沉降值增加的过表达1Bx7谷蛋白小麦近等基因系的培育

Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values.

作者信息

Lee Myoung-Hui, Choi Changhyun, Kim Kyeong-Hoon, Son Jae-Han, Lee Go-Eun, Choi Jun-Yong, Kang Chon-Sik, Shon Jiyoung, Ko Jong-Min, Kim Kyeong-Min

机构信息

Wheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of Korea.

出版信息

Plants (Basel). 2023 Mar 9;12(6):1244. doi: 10.3390/plants12061244.

DOI:10.3390/plants12061244
PMID:36986938
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10051937/
Abstract

Overexpression of Glu-1Bx7 via allele 1Bx7 significantly contributes to high dough strength in some wheat varieties and is useful for improving wheat quality. However, the proportion of wheat varieties containing Bx7 is quite low. In this study, four cultivars containing 1Bx7 were selected, and among the selected varieties, Chisholm (1Ax2*, 1Bx7 + 1By8*, and 1Dx5 + 1Dx10) was crossed with Keumkang, a wheat variety that contains 1Bx7 (1Ax2*, 1Bx7 + 1By8, and 1Dx5 + 1Dx10). SDS-PAGE and UPLC analyses showed that the expression of the high-molecular-weight glutenin subunit (HMW-GS) 1Bx7 was significantly higher in NILs (1Ax2*, 1Bx7 + 1By8*, and 1Dx5 + 1Dx10) compared with that in Keumkang. Wheat quality was analyzed with near infrared reflectance spectroscopy by measuring the protein content and SDS-sedimentation of NILs. The protein content of NILs (12.94%) was 21.65% higher than that of Chisholm (10.63%) and 4.54% higher than that of Keumkang (12.37%). In addition, the SDS-sedimentation value of NILs (44.29 mL) was 14.97% and 16.44% higher than that of Keumkang (38.52 mL) and Chisholm (38.03 mL), respectively. This study predicts that the quality of domestic wheat can be improved by crossbreeding with 1Bx7-containing cultivars.

摘要

通过1Bx7等位基因过表达Glu-1Bx7对某些小麦品种的高面团强度有显著贡献，有助于改善小麦品质。然而，含有Bx7的小麦品种比例相当低。本研究选择了四个含有1Bx7的品种，在所选品种中，奇泽姆（1Ax2*、1Bx7 + 1By8和1Dx5 + 1Dx10）与含有1Bx7的小麦品种锦康（1Ax2、1Bx7 + 1By8和1Dx5 + 1Dx10）杂交。SDS-PAGE和UPLC分析表明，与锦康相比，近等基因系（1Ax2*、1Bx7 + 1By8*和1Dx5 + 1Dx10）中高分子量谷蛋白亚基（HMW-GS）1Bx7的表达显著更高。通过测量近等基因系的蛋白质含量和SDS沉降值，用近红外反射光谱法分析小麦品质。近等基因系的蛋白质含量（12.94%）比奇泽姆（10.63%）高21.65%，比锦康（12.37%）高4.54%。此外，近等基因系的SDS沉降值（44.29 mL）分别比锦康（38.52 mL）和奇泽姆（38.03 mL）高14.97%和16.44%。本研究预测，通过与含有1Bx7的品种杂交可以提高国产小麦的品质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/4f38853356ca/plants-12-01244-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/deb01607658a/plants-12-01244-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/19d16e3ee7bf/plants-12-01244-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/f2dfcdfaf43c/plants-12-01244-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/a3c6d3a9db59/plants-12-01244-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/bbd4fa09d63c/plants-12-01244-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/7e2e53e77cb2/plants-12-01244-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/4f38853356ca/plants-12-01244-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/deb01607658a/plants-12-01244-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/19d16e3ee7bf/plants-12-01244-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/f2dfcdfaf43c/plants-12-01244-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/a3c6d3a9db59/plants-12-01244-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/bbd4fa09d63c/plants-12-01244-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/7e2e53e77cb2/plants-12-01244-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa30/10051937/4f38853356ca/plants-12-01244-g007.jpg

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