染色质重塑因子将DNA甲基化作为靶点，以正向调控[具体植物名称]中的顶端优势。（注：原文中缺失植物名称，翻译时做了补充说明）

The Chromatin Remodeling Factor Targets DNA Methylation to Positively Regulate Apical Dominance in .

作者信息

Zhu Wei, Xie Zhengqing, Chu Zhenni, Ding Yakun, Shi Gongyao, Chen Weiwei, Wei Xiaochun, Yuan Yuxiang, Wei Fang, Tian Baoming

机构信息

Henan International Joint Laboratory of Crop Gene Resource and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.

Institute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, China.

出版信息

Plants (Basel). 2023 Mar 20;12(6):1384. doi: 10.3390/plants12061384.

DOI:10.3390/plants12061384

PMID:36987072

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10051476/

Abstract

The SHPRH (SNF2, histone linker, PHD, RING, helicase) subfamily belonging to ATP-dependent chromatin remodeling factor is the effective tumor-suppressor, which can polyubiquitinate PCNA (proliferating cell nuclear antigen) and participate in post-replication repair in human. However, little is known about the functions of SHPRH proteins in plants. In this study, we identified a novel SHPRH member and obtained -silenced transgenic . In contrast to wild-type plants, transgenic plants exhibited a released apical dominance phenotype with semi-dwarfism and multiple lateral branches. Furthermore, a global alteration of DNA methylation in the main stem and bud appeared after silencing of . Based on the GO (gene ontology) functional annotation and KEGG (Kyoto encyclopedia of genes and genomes) pathway analysis, the plant hormone signal transduction pathway was clearly enriched. In particular, we found a significant increase in the methylation level of auxin-related genes in the stem, whereas auxin- and cytokinin-related genes were hypomethylated in the bud of transgenic plants. In addition, further qRT-PCR (quantitative real-time PCR) analysis revealed that DNA methylation level always had an opposite trend with gene expression level. Considered together, our findings indicated that suppression of expression triggered the methylation divergence of hormone-related genes and subsequently affected transcription levels to regulate the apical dominance in .

摘要

属于ATP依赖型染色质重塑因子的SHPRH（SNF2、组蛋白连接蛋白、PHD、RING、解旋酶）亚家族是一种有效的肿瘤抑制因子，它能使增殖细胞核抗原（PCNA）多聚泛素化并参与人类的复制后修复。然而，人们对SHPRH蛋白在植物中的功能知之甚少。在本研究中，我们鉴定出一个新的SHPRH成员，并获得了其沉默的转基因植株。与野生型植株相比，转基因植株表现出解除顶端优势的表型，具有半矮化和多个侧枝。此外，在该基因沉默后，主茎和芽中的DNA甲基化出现了全局改变。基于基因本体（GO）功能注释和京都基因与基因组百科全书（KEGG）通路分析，植物激素信号转导通路明显富集。特别是，我们发现茎中生长素相关基因的甲基化水平显著增加，而转基因植株芽中生长素和细胞分裂素相关基因发生了低甲基化。此外，进一步的定量实时PCR（qRT-PCR）分析表明，DNA甲基化水平与基因表达水平总是呈相反趋势。综合来看，我们的研究结果表明，该基因表达的抑制引发了激素相关基因的甲基化差异，随后影响转录水平以调节该植物的顶端优势。