从[具体来源]中天然深共熔溶剂提取生物活性色素及静电纺丝能力评估。 （注：原文中“from”后面似乎缺少具体内容）
Natural Deep Eutectic Solvent Extraction of Bioactive Pigments from and Electrospinning Ability Assessment.
作者信息
Martins Rodrigo, Mouro Cláudia, Pontes Rita, Nunes João, Gouveia Isabel
机构信息
Association BLC3-Technology and Innovation Campus, Centre Bio R & D Unit, 3405-155 Oliveira do Hospital, Portugal.
FibEnTech Research Unit, Faculty of Engineering, University of Beira Interior, 6200-001 Covilhã, Portugal.
出版信息
Polymers (Basel). 2023 Mar 22;15(6):1574. doi: 10.3390/polym15061574.
The first ever nanofibers produced by the electrospinning of polyvinyl alcohol (PVA) and extracts are presented in this article. extracts were obtained by ultrasound-assisted extraction (UAE) using two different solvents: a glucose/glycerol-based natural deep eutectic solvent (NADES) and water. Through spectrophotometry analysis, it was possible to determine the pigment yield of the extractions for both extracts: phycocyanin = 3.79 ± 0.05 mg/g of dry biomass (DB); chlorophylls = 0.24 ± 0.05 mg/g DB; carotenoids = 0.13 ± 0.03 mg/g DB for the NADES/Spirulina extracts, and phycocyanin = 0.001 ± 0.0005 mg/g DB; chlorophylls = 0.10 ± 0.05 mg/g DB; carotenoids = 0.20 ± 0.05 mg/g DB for water/Spirulina extracts. Emulsions were formed by mixing the microalgae extracts in PVA (9%, /) at different concentrations: 5, 20, 40, and 50% (/). Electrospinning was carried out at the following conditions: 13 cm of distance to collector; 80 kV of applied voltage; and 85 rpm of electrode rotation. After the nanofibers were collected, they were checked under a scanning electron microscope (SEM). ImageJ was also used to determine fiber diameter and frequency. SEM results showed the formation of nanofibers for 5 and 20% (/) of NADES/Spirulina extract content in the electrospinning emulsions, presenting diameters of 423.52 ± 142.61 nm and 680.54 ± 271.92 nm, respectively. FTIR confirmed the presence of the NADES extracts in the nanofibers produced. Overall, the nanofibers produced showed promising antioxidant activities, with the NADES/Spirulina- and PVA-based nanofibers displaying the highest antioxidant activity (47%). The highest antimicrobial activity (89.26%) was also obtained by the NADES/Spirulina and PVA nanofibers (20%, /). Principal Component Analysis (PCA) revealed positive correlations between both the antioxidant and antimicrobial activities of the electrospun nanofibers, and extract content in the emulsions. Moreover, PCA also indicated positive correlations between the viscosity and conductivity of the emulsions and the diameter of the nanofibers produced.
本文介绍了通过静电纺丝聚乙烯醇（PVA）和提取物首次制备的纳米纤维。提取物通过超声辅助提取（UAE）获得，使用两种不同的溶剂：基于葡萄糖/甘油的天然低共熔溶剂（NADES）和水。通过分光光度法分析，可以确定两种提取物的色素提取率：对于NADES/螺旋藻提取物，藻蓝蛋白=3.79±0.05mg/g干生物量（DB）；叶绿素=0.24±0.05mg/g DB；类胡萝卜素=0.13±0.03mg/g DB；对于水/螺旋藻提取物，藻蓝蛋白=0.001±0.0005mg/g DB；叶绿素=0.10±0.05mg/g DB；类胡萝卜素=0.20±0.05mg/g DB。通过将微藻提取物与不同浓度（5%、20%、40%和50%，v/v）的PVA（9%，w/v）混合形成乳液。静电纺丝在以下条件下进行：距收集器13cm；施加电压80kV；电极旋转速度85rpm。收集纳米纤维后，在扫描电子显微镜（SEM）下进行检查。还使用ImageJ来确定纤维直径和频率。SEM结果表明，在静电纺丝乳液中，当NADES/螺旋藻提取物含量为5%和20%（v/v）时形成了纳米纤维，其直径分别为423.52±142.61nm和680.54±271.92nm。傅里叶变换红外光谱（FTIR）证实了所制备的纳米纤维中存在NADES提取物。总体而言，所制备的纳米纤维显示出有前景的抗氧化活性，基于NADES/螺旋藻和PVA的纳米纤维表现出最高的抗氧化活性（47%）。NADES/螺旋藻和PVA纳米纤维（20%，v/v）也获得了最高的抗菌活性（89.26%）。主成分分析（PCA）揭示了静电纺纳米纤维的抗氧化和抗菌活性与乳液中提取物含量之间的正相关关系。此外，PCA还表明乳液的粘度和电导率与所制备的纳米纤维直径之间存在正相关关系。
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