Avilés-Betanzos Kevin Alejandro, Oney-Montalvo Julio Enrique, Cauich-Rodríguez Juan Valerio, González-Ávila Marisela, Scampicchio Matteo, Morozova Ksenia, Ramírez-Sucre Manuel Octavio, Rodríguez-Buenfil Ingrid Mayanin
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Subsede Sureste, Tablaje Catastral 31264, Km. 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico Tecnológico de Yucatán, Sierra Papacal 97302, Mexico.
Centro de Investigación Científica de Yucatán, Unidad de Materiales, Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida 97205, Mexico.
Plants (Basel). 2022 Aug 6;11(15):2060. doi: 10.3390/plants11152060.
Habanero pepper leaves and stems (by-products) have been traditionally considered waste; however, bioactive compounds such as polyphenols, vitamin C and carotenoids have been identified that can be used for formulation of nutraceuticals or functional foods. Furthermore, the extraction of these bioactive compounds by using environmentally friendly methods and solvents is desirable. Thus, the aim of this study was to assess the antioxidant capacity, total polyphenol content (TPC), the phenolic profile and vitamin C content in extracts obtained from by-products (stems and leaves) of two varieties (Mayapan and Jaguar) of habanero pepper by ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES). The results showed that NADES leads to extracts with significantly higher TPC, higher concentrations of individual polyphenols (gallic acid, protocatechuic acid, chlorogenic acid, cinnamic acid, coumaric acid), vitamin C and, finally, higher antioxidant capacity (9.55 ± 0.02 eq mg Trolox/g DM) than UAE extraction performed with methanol as the solvent. The association of individual polyphenols with NADES was confirmed by principal component analysis (PCA). Overall, NADES is an innovative and promising "green" extraction technique that can be applied successfully for the extraction of phenolic compounds from habanero pepper by-products.
哈瓦那辣椒的叶子和茎（副产品）传统上被视为废弃物；然而，已鉴定出诸如多酚、维生素C和类胡萝卜素等生物活性化合物，它们可用于营养保健品或功能性食品的配方。此外，采用环境友好型方法和溶剂来提取这些生物活性化合物是很有必要的。因此，本研究的目的是通过使用天然低共熔溶剂（NADES）的超声辅助提取（UAE），评估从哈瓦那辣椒两个品种（玛雅潘和美洲豹）的副产品（茎和叶）中获得的提取物的抗氧化能力、总多酚含量（TPC）、酚类物质谱和维生素C含量。结果表明，与以甲醇为溶剂进行的UAE提取相比，NADES得到的提取物具有显著更高的TPC、更高浓度的单个多酚（没食子酸、原儿茶酸、绿原酸、肉桂酸、香豆酸）、维生素C，最终还具有更高的抗氧化能力（9.55±0.02当量毫克Trolox/克干物质）。通过主成分分析（PCA）证实了单个多酚与NADES之间的关联。总体而言，NADES是一种创新且有前景的“绿色”提取技术，可成功应用于从哈瓦那辣椒副产品中提取酚类化合物。
