de Boer P, Searle A G, van der Hoeven F A, de Rooij D G, Beechey C V
Chromosoma. 1986;93(4):326-36. doi: 10.1007/BF00327591.
In order to clarify the relationship between meiotic pairing and progress of spermatogenesis, an analysis of male meiotic pairing was carried out in four reciprocal translocation heterozygotes and two double heterozygotes for two semi-identical reciprocal translocations. The reciprocal translocations were chosen to range from fertility (T70H/+) through almost complete sterility (T31H/+) to complete sterility (T32H/+, T42/H+). If meiotic pairing in the translocation multivalent was incomplete, it concerned terminal or probably more often proximal chromosome segments (Chain IV). If both segments failed to pair the multivalent symbol is Chain III + I. Complete pairing is symbolized by Ring IV. To contrast and complement observations of this type, the double heterozygotes were introduced. Males of this type in theory possess two heteromorphic bivalents with a central area of incomplete meiotic pairing (loop formation). Of the T70H/T1Wa double heterozygotes, 36% of the males are capable of inducing at least one decidual reaction in two females whereas for T26H/T2Wa, 79% of the males can do so. For the reciprocal translocations, it was found that proximity of the multivalent to the sex bivalent during pachytene increased in the order Ring IV, Chain IV, Chain III + I. The degree of spermatogenic impairment as measured from cell counts in histological sections and tubular whole mounts, is positively related to the frequency of proximity between the sex chromosomes and the translocation multivalent and thus to lack of meiotic pairing within the multivalent. The meiotic pairing analysis of the double heterozygotes yielded the following findings. For the long heteromorphic bivalents a true loop was never seen in T70H/T1Wa and only rarely observed in T26H/T2Wa. Small marker bivalents of both types were usually recognizable by the following criteria: pairing confined to distal or proximal segments, both distal and proximal segments pairing and loop formation and pairing covering the entire length of both "homologues" but the longer one often with a "thickened" lateral element. The same positive correlation between the absence of pairing (proximal, distal or central) and the proximity of the small marker bivalent synaptonemal complex to the sex bivalent has been found as for unpaired segments within reciprocal translocation multivalents. One unexpected finding was the occurrence of diploid spermatids and spermatozoa especially in T32H/+ males (70-91%) but also in T31H/+ (3-39%).
为了阐明减数分裂配对与精子发生进程之间的关系，对四个相互易位杂合子和两个涉及两个半同源相互易位的双杂合子进行了雄性减数分裂配对分析。所选择的相互易位涵盖了从可育（T70H/+）到几乎完全不育（T31H/+）再到完全不育（T32H/+, T42/H+）的范围。如果易位多价体中的减数分裂配对不完全，涉及的是末端或可能更常见的近端染色体片段（链式IV）。如果两个片段都未配对，多价体符号为链式III + I。完全配对用环状IV表示。为了对比和补充这类观察结果，引入了双杂合子。理论上，这类雄性具有两个异形二价体，其减数分裂配对的中心区域存在不完全配对（环形成）。在T70H/T1Wa双杂合子中，36%的雄性能够在两只雌性中诱导至少一次蜕膜反应，而对于T26H/T2Wa，79%的雄性能够做到。对于相互易位，发现在粗线期多价体与性二价体的接近程度按环状IV、链式IV、链式III + I的顺序增加。从组织学切片和管状整装片中的细胞计数测量的生精损伤程度，与性染色体和易位多价体之间接近的频率呈正相关，因此与多价体内减数分裂配对的缺乏呈正相关。双杂合子的减数分裂配对分析得出了以下结果。对于长异形二价体，在T70H/T1Wa中从未见过真正的环，在T26H/T2Wa中也很少观察到。两种类型的小标记二价体通常可通过以下标准识别：配对局限于远端或近端片段、远端和近端片段都配对且形成环以及配对覆盖两个“同源染色体”的全长，但较长的一个通常有一个“增厚”的侧元件。对于相互易位多价体内未配对的片段，在小标记二价体联会复合体与性二价体的接近程度和未配对（近端、远端或中心）之间发现了相同的正相关。一个意外的发现是二倍体精子细胞和精子的出现，特别是在T32H/+雄性中（70 - 91%），但在T31H/+中也有（3 - 39%）。
