Department of Virology, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
Institute of Biotechnology, DNA Sequencing and Genomics Laboratory, University of Helsinki, Helsinki, Finland.
J Infect Dis. 2023 Oct 3;228(7):829-833. doi: 10.1093/infdis/jiad083.
Progressive multifocal leukoencephalopathy (PML) is a severe neurological condition caused by reactivation of JC polyomavirus (JCPyV) in immunosuppression. Asymptomatic JCPyV persists in peripheral tissues. Upon reactivation, neurotropic rearrangements may emerge, and the virus gains access to the brain. To assess the mechanisms of PML pathogenesis, brain tissue material from PML patients was collected for small RNA sequencing. Upregulation of 8 microRNAs (miRNAs) in PML brain was validated using quantitative microRNA polymerase chain reaction (PCR). Bioinformatics tools were utilized to identify major associations of the upregulated miRNAs: neuroinflammation and blood-brain barrier disruption. The results indicate involvement of human miRNA regulation in PML pathogenesis.
进行性多灶性白质脑病（PML）是由 JC 多瘤病毒（JCPyV）在免疫抑制下重新激活引起的严重神经疾病。无症状的 JCPyV 存在于外周组织中。重新激活后，神经嗜性重排可能出现，病毒进入大脑。为了评估 PML 发病机制的机制，从 PML 患者的脑组织中收集了小 RNA 测序。使用定量 microRNA 聚合酶链反应（PCR）验证了 PML 脑中小 RNA（miRNA）的 8 种上调。利用生物信息学工具鉴定上调 miRNA 的主要关联：神经炎症和血脑屏障破坏。结果表明，人类 miRNA 调节参与了 PML 的发病机制。
