银屑病患者血清 LL-37 和炎症细胞因子水平。

Serum LL-37 and inflammatory cytokines levels in psoriasis.

机构信息

Department of Laboratory Medicine, Guangxi Academy of Medical Sciences, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.

Department of Dermatology and Venereology, Guangxi Academy of Medical Sciences, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.

出版信息

Immun Inflamm Dis. 2023 Mar;11(3):e802. doi: 10.1002/iid3.802.

DOI:10.1002/iid3.802

PMID:36988247

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10013411/

Abstract

BACKGROUND

Psoriasis (PsO) is a T-cell-associated inflammatory autoimmune dermatitis. Leucine leucine-37 (LL-37) is upregulated in PsO patients and correlated with the area and severity of PsO. However, the exact relation between LL-37 and T cell-associated inflammation is not well understood. It is very important to clarify the relationship between LL-37 and inflammatory response for clinical diagnosis and treatment of PsO. This study investigated the serum levels of LL-37 and inflammatory cytokines, as well as correlations between them in PsO patients, which aimed to provide new ideas for the diagnosis and treatment of PsO.

METHODS

PsO patients (n = 50) and healthy volunteers (n = 33) were recruited in this study. Skin specimens were stained with hematoxylin and eosin (H&E). The serum levels of LL-37, T-helper type 1 (Th1, IFN-γ), T-helper type 17 (Th17, IL-17), T-helper type 22 (Th22, IL-22), and T-helper type 2 cytokines (Th2, IL-4) were assessed by enzyme-linked immunosorbent assay. Some of the patients were re-recruited after treatment to evaluate LL-37 and cytokines levels.

RESULTS

Pathological changes were observed in PsO skin lesions. LL-37, IFN-γ, IL-17, and IL-22 serum levels were much higher in PsO patients than those in healthy volunteers (p < .001), and posttreatment reduction was observed in five patients. However, no remarkable difference in IL-4 level (p > .05) was found. LL-37 level was positively correlated with IFN-γ, IL-17, and IL-22 levels (p < .001) in PsO patients.

CONCLUSION

LL-37 expression was significantly associated with inflammatory response, which may provide us new ideas for diagnosing and monitoring disease activity of PsO.

摘要

背景

银屑病（PsO）是一种 T 细胞相关的炎症性自身免疫性皮肤病。亮氨酸亮氨酸-37（LL-37）在 PsO 患者中上调，并与 PsO 的面积和严重程度相关。然而，LL-37 与 T 细胞相关炎症的确切关系尚不清楚。阐明 LL-37 与炎症反应之间的关系对于 PsO 的临床诊断和治疗非常重要。本研究调查了 PsO 患者血清中 LL-37 和炎症细胞因子的水平及其之间的相关性，旨在为 PsO 的诊断和治疗提供新的思路。

方法

本研究招募了 50 名 PsO 患者和 33 名健康志愿者。苏木精和伊红（H&E）染色皮肤标本。通过酶联免疫吸附试验评估血清 LL-37、辅助性 T 细胞 1（Th1、IFN-γ）、辅助性 T 细胞 17（Th17、IL-17）、辅助性 T 细胞 22（Th22、IL-22）和辅助性 T 细胞 2 细胞因子（Th2、IL-4）的水平。一些患者在治疗后重新招募，以评估 LL-37 和细胞因子水平。

结果

在 PsO 皮肤病变中观察到病理变化。与健康志愿者相比，PsO 患者的血清 LL-37、IFN-γ、IL-17 和 IL-22 水平明显更高（p <.001），并且在五名患者中观察到治疗后降低。然而，IL-4 水平没有明显差异（p >.05）。在 PsO 患者中，LL-37 水平与 IFN-γ、IL-17 和 IL-22 水平呈正相关（p <.001）。