He Anqi, Zheng Shaoluan, Luan Wenjie, Wang Lu, Qian Leqi, Qi Fazhi, Feng Zihao
From the Department of Plastic Surgery, Zhongshan Hospital (Xiamen), Fudan University.
Department of Plastic Surgery, Zhongshan Hospital, Fudan University.
Plast Reconstr Surg. 2023 Nov 1;152(5):1023-1033. doi: 10.1097/PRS.0000000000010458. Epub 2023 Mar 28.
Adipose-derived stromal vascular fraction (SVF) and mesenchymal stem cells have been proven to reduce the effects of skin photoaging. However, there is no standardized protocol for their preparation. This study aimed to investigate the skin rejuvenation potential of micronized fat, obtained using a novel device attached with a trifoliate blade, in the ultraviolet B (UV-B)-induced human dermal fibroblast model.
Micronized fat was prepared to obtain adipose-derived SVF, and the adipose-derived mesenchymal stem cell-to-SVF ratio was determined by flow cytometry. The UV-B-induced human dermal fibroblasts model was constructed to identify the characteristics of the human dermal fibroblasts using vimentin and S-100 immunostaining, observe their morphology, and measure the levels of photoaging-related factors. After the previous steps were completed, different cell groups were co-cultured with UV-B-induced human dermal fibroblasts, and the extent of improvement of photoaging was evaluated.
Micronized fat had a higher adipose-derived mesenchymal stem cell-to-SVF ratio than the control fat preparations. The UV-B-induced human dermal fibroblasts model showed lowered levels of type I collagen and transforming growth factor-β and increased expression of matrix metalloproteinases (MMPs), which are the characteristics of photoaging in normal human dermal fibroblasts. Compared with different cell groups co-cultured with UV-B-induced human dermal fibroblasts, micronized fat could lower the expression of MMPs and increase the level of type I collagen but lower the level of transforming growth factor-β.
Obtaining micronized fat is more effortless and clinically safer. Micronized fat has an antiphotoaging effect by inhibiting the expression of MMPs by means of the mitogen-activated protein kinases signaling pathway.
The authors' work has potential clinical applications in fat grafting for facial rejuvenation.
脂肪来源的基质血管成分（SVF）和间充质干细胞已被证明可减轻皮肤光老化的影响。然而，其制备尚无标准化方案。本研究旨在探讨使用附有三叶刀片的新型装置获得的微化脂肪在紫外线B（UV-B）诱导的人真皮成纤维细胞模型中的皮肤年轻化潜力。
制备微化脂肪以获得脂肪来源的SVF，并通过流式细胞术测定脂肪来源的间充质干细胞与SVF的比例。构建UV-B诱导的人真皮成纤维细胞模型，使用波形蛋白和S-100免疫染色鉴定人真皮成纤维细胞的特征，观察其形态，并测量光老化相关因子的水平。在完成上述步骤后，将不同细胞组与UV-B诱导的人真皮成纤维细胞共培养，并评估光老化的改善程度。
微化脂肪的脂肪来源间充质干细胞与SVF的比例高于对照脂肪制剂。UV-B诱导的人真皮成纤维细胞模型显示I型胶原蛋白和转化生长因子-β水平降低，基质金属蛋白酶（MMPs）表达增加，这是正常人真皮成纤维细胞光老化的特征。与与UV-B诱导的人真皮成纤维细胞共培养的不同细胞组相比，微化脂肪可降低MMPs的表达，增加I型胶原蛋白水平，但降低转化生长因子-β水平。
获得微化脂肪更简便且临床安全性更高。微化脂肪通过丝裂原活化蛋白激酶信号通路抑制MMPs的表达而具有抗光老化作用。
作者的工作在用于面部年轻化的脂肪移植中具有潜在的临床应用价值。
