From the Department of Plastic Surgery, Zhongshan Hospital, Fudan University.
Plast Reconstr Surg. 2020 Mar;145(3):712-720. doi: 10.1097/PRS.0000000000006607.
Autologous fat grafting has long been an essential technique in cosmetic and reconstructive surgery. Here, the authors report the advantages of a new device for preparing micronized fat, and they also investigated the therapeutic potential of micronized fat against ultraviolet B-induced photoaging.
Micronized fat aliquots were prepared through a connector device with trifoliate blades. The histologic structure and viability of the prepared fat samples were evaluated by calcein AM/propidium iodide staining. The levels of growth factor were measured by enzyme-linked immunosorbent assay, and flow cytometry was used to detect the ratio of adipose-derived mesenchymal stem cells to stromal vascular fraction. The authors also evaluated the effects of micronized fat transplantation through immunohistochemistry and Masson trichrome staining in an animal model of photoaging.
The micronized fat had a normal histologic structure and viable adipocytes. It had a higher level of hepatocyte growth factor compared with the control group, and its ratio of adipose-derived mesenchymal stem cells to stromal vascular fraction was also higher than in the control fat preparations. Transplantation of micronized fat preparations in the animal model of photoaging produced increased skin neovascularization, epidermal cell proliferation, and dermal collagen density.
The authors' results demonstrated that the novel device produced micronized fat easily, which can condense adipose tissue. This micronized fat was easy to use with smaller cannulas. It mitigated the signs of cutaneous photoaging and was superior to control fat. Contrary to previous reports, normal histologic structures and viable adipocytes were noted in the micronized fat.
自体脂肪移植长期以来一直是美容和重建外科的一项基本技术。作者在这里报告了一种新型微化脂肪制备装置的优势，并研究了微化脂肪对紫外线 B 诱导的光老化的治疗潜力。
通过三叶刀片连接器装置制备微化脂肪等分试样。通过钙黄绿素 AM/碘化丙啶染色评估制备的脂肪样本的组织学结构和活力。通过酶联免疫吸附试验测量生长因子水平，并用流式细胞术检测脂肪源性间充质干细胞与基质血管部分的比例。作者还通过免疫组织化学和 Masson 三色染色评估了光老化动物模型中微化脂肪移植的效果。
微化脂肪具有正常的组织学结构和存活的脂肪细胞。与对照组相比，它具有更高水平的肝细胞生长因子，其脂肪源性间充质干细胞与基质血管部分的比例也高于对照组的脂肪制备物。在光老化动物模型中移植微化脂肪制剂可增加皮肤新生血管、表皮细胞增殖和真皮胶原密度。
作者的结果表明，新型装置可轻松制备微化脂肪，可浓缩脂肪组织。这种微化脂肪使用较小的套管更容易操作。它减轻了皮肤光老化的迹象，优于对照脂肪。与之前的报告相反，在微化脂肪中观察到正常的组织学结构和存活的脂肪细胞。
