Department of Chemical Engineering & Technology, Indian Institute of Technology (BHU), Uttar Pradesh, Varanasi, 221005, India.
Department of Chemical Engineering, Indian Institute of Petroleum and Energy (IIPE), Visakhapatnam, 530003, Andhra Pradesh, India.
Appl Biochem Biotechnol. 2023 Sep;195(9):5267-5279. doi: 10.1007/s12010-023-04425-w. Epub 2023 Mar 29.
The objective of this work was to develop a low-cost and efficient biocarrier for biodegradation of azo dye (i.e., Congo red (CR) dye). The potential bacterial species, i.e., Lysinibacillus fusiformis KLM1 and Lysinibacillus macrolides KLM2, were isolated from the dye-contaminated site. These bacterial species were immobilized onto the polypropylene-polyurethane foam (PP-PUF) and employed in a moving bed biofilm reactor (MBBR) for the treatment of CR dye. The effectiveness of the MBBR was investigated by operating the bioreactor in a continuous mode at various initial CR dye concentrations (50-250 mg/L) for 113 days. The removal efficiency was found in the range of 88.4-64.6% when the initial dye concentration was varied from 50 to 250 mg/L. The maximum elimination capacity (EC) of 213.18 mg/L.d was found at 250 mg/L of CR dye concentration. In addition, the CR dye utilization rate in the MBBR was studied by using two kinetics, namely, first-order and second-order (Grau) models. The high regression coefficients (R > 0.97) and the satisfactory root mean square (RMSE) values (0.00096-0.02610) indicated the reasonable prediction of CR dye degradation rate by the Grau model.
本工作旨在开发一种低成本、高效的生物载体，用于偶氮染料（如刚果红（CR）染料）的生物降解。从染料污染场地中分离出潜在的细菌物种，即 Fusiformis Lysinibacillus KLM1 和 Macrolides Lysinibacillus KLM2，并将其固定在聚丙烯-聚氨酯泡沫（PP-PUF）上，然后在移动床生物膜反应器（MBBR）中用于处理 CR 染料。通过在连续模式下以不同的初始 CR 染料浓度（50-250 mg/L）运行生物反应器 113 天，研究了 MBBR 的有效性。当初始染料浓度从 50 到 250 mg/L 变化时，去除效率在 88.4-64.6%范围内。在 250 mg/L 的 CR 染料浓度下，发现最大消除容量（EC）为 213.18 mg/L.d。此外，通过使用两种动力学，即一级和二级（Grau）模型，研究了 MBBR 中 CR 染料的利用率。高回归系数（R>0.97）和令人满意的均方根（RMSE）值（0.00096-0.02610）表明 Grau 模型能够合理地预测 CR 染料的降解速率。
