用聚氨酯泡沫（PUF）和海藻酸钠珠粒填充生物反应器，并固定枯草芽孢杆菌 M3，研究苯的生物降解和动力学。
Biodegradation and kinetic study of benzene in bioreactor packed with PUF and alginate beads and immobilized with Bacillus sp. M3.
机构信息
Department of Chemical Engineering & Technology, IIT (BHU), Varanasi, UP 221005, India.
Department of Chemical Engineering & Technology, IIT (BHU), Varanasi, UP 221005, India.
出版信息
Bioresour Technol. 2017 Oct;242:92-100. doi: 10.1016/j.biortech.2017.03.167. Epub 2017 Mar 30.
Benzene removal in free and immobilized cells on polyurethane foam (PUF) and polyvinyl alcohol (PVA)-alginate beads was studied using an indigenous soil bacterium Bacillus sp. M3 isolated from petroleum-contaminated soil. The important process parameters (pH, temperature and inoculums size) were optimized and found to be 7, 37°C and 6.0×10CFU/mL, respectively. Benzene removals were observed to be 70, 84 and 90% within 9days in a free cell, immobilized PVA-alginate beads and PUF, respectively under optimum operating conditions. FT-IR and GC-MS analysis confirm the presence of phenol, 1,2-benzenediol, hydroquinone and benzoate as metabolites. The important kinetic parameter ratios (µ/K; L/mg·day calculated using Monod model was found to be 0.00123 for free cell, 0.00159 for immobilized alginate beads and 0.002016 for immobilized PUF. Similarly inhibition constants (K; mg/L) calculated using Andrew-Haldane model was found to be 435.84 for free cell, 664.25 for immobilized alginate beads and 724.93 for immobilized PUF.
采用从石油污染土壤中分离得到的本土土壤细菌 Bacillus sp. M3，研究了游离细胞和固定在聚氨酯泡沫（PUF）和聚乙烯醇（PVA）-海藻酸钠珠上的细胞对苯的去除效果。优化了重要的工艺参数（pH、温度和接种量），分别为 7、37°C 和 6.0×10CFU/mL。在最佳操作条件下，游离细胞、固定化 PVA-海藻酸钠珠和 PUF 分别在 9 天内观察到苯的去除率分别为 70%、84%和 90%。FT-IR 和 GC-MS 分析证实存在苯酚、1,2-苯二酚、对苯二酚和苯甲酸作为代谢物。使用 Monod 模型计算的重要动力学参数比（µ/K；L/mg·天）分别为游离细胞 0.00123、固定化海藻酸钠珠 0.00159 和固定化 PUF 0.002016。同样，使用 Andrew-Haldane 模型计算的抑制常数（K；mg/L）分别为游离细胞 435.84、固定化海藻酸钠珠 664.25 和固定化 PUF 724.93。
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