Enzyme Technology and Protein Bioinformatics Laboratory, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India.
Enzyme Technology and Protein Bioinformatics Laboratory, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India.
Bioresour Technol. 2023 Jul;379:128974. doi: 10.1016/j.biortech.2023.128974. Epub 2023 Mar 27.
Recent advances in metabolic engineering have made cyanobacteria emerge as promising and attractive microorganisms for sustainable production, by exploiting their natural capability for producing metabolites. The potential of metabolically engineered cyanobacterium would depend on its source-sink balance in the same way as other phototrophs. In cyanobacteria, the amount of light energy harvested (Source) is incompletely utilized by the cell to fix carbon (sink) resulting in wastage of the absorbed energy causing photoinhibition and cellular damage leading to lowered photosynthetic efficiency. Although regulatory pathways like photo-acclimation and photoprotective processes can be helpful unfortunately they limit the cell's metabolic capacity. This review describes approaches for source-sink balance and engineering heterologous metabolic sinks in cyanobacteria for enhanced photosynthetic efficiency. The advances for engineering additional metabolic pathways in cyanobacteria are also described which will provide a better understanding of the cyanobacterial source-sink balance and approaches for efficient cyanobacterial strains for valuable metabolites.
近年来，代谢工程的进展使得蓝藻作为一种有前途和有吸引力的微生物，通过利用其天然生产代谢物的能力，在可持续生产方面崭露头角。代谢工程化蓝藻的潜力将取决于其源-汇平衡，这与其他光合生物相同。在蓝藻中，细胞不完全利用收获的光能（源）来固定碳（汇），导致吸收的能量浪费，从而引起光抑制和细胞损伤，导致光合作用效率降低。虽然像光驯化和光保护过程等调节途径可能会有所帮助，但不幸的是，它们限制了细胞的代谢能力。本文综述了蓝藻中源-汇平衡和工程异源代谢汇的方法，以提高光合作用效率。还描述了在蓝藻中工程化额外代谢途径的进展，这将更好地理解蓝藻的源-汇平衡以及高效蓝藻菌株生产有价值代谢物的方法。
