Research Institute for Future Food, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong; Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University, Linyi, China.
Research Institute for Future Food, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Int J Biol Macromol. 2023 May 15;237:124216. doi: 10.1016/j.ijbiomac.2023.124216. Epub 2023 Mar 27.
A hyphenated process, autoclaving followed by ultrasonication (AU), was evaluated for efficient extraction of polysaccharides (PS) from Lentinula edodes (Shiitake) mushroom. The PS yield (w/w) was 8.44 % from hot-water extraction (HWE), 11.01 % by autoclaving extraction (AE), and 16.3 % by AUE. The AUE water extract was subject to fractional precipitation in four-steps with increasing ethanol concentration of 40 %, 50 %, 70 % and 80 % (v/v), yielding four PS fractions in descending molecular weight (MW), PS40 > PS50 > PS70 > PS80. All the four PS fractions were composed of four monosaccharide residues, mannose (Man), glucose (Glc) and galactose (Gal) but in different mole ratios. The PS40 fraction with the highest average MW (4.98 × 10) was the most abundant fraction, accounting for 64.4 % of the total PS mass and also had the highest glucose molar ratio (~80 %). PS40 also most significantly enhanced the NO, and ROS generation and phagocytic activity in RAW 264.7 cells. The results proved that AUE followed by fractional ethanol precipitation is an efficient strategy with reduced solvent expenditure for isolation of the major immunostimulatory PS from L. edodes mushroom.
超声辅助提取-高压蒸煮法从香菇中高效提取多糖的研究
采用超声辅助提取-高压蒸煮法（AU）对香菇进行处理，以评估其从香菇中高效提取多糖（PS）的能力。热水提取（HWE）的 PS 得率（w/w）为 8.44%，高压蒸煮提取（AE）的 PS 得率为 11.01%，AU 的 PS 得率为 16.3%。AU 水提取物采用分步沉淀法，用 40%、50%、70%和 80%（v/v）的乙醇浓度进行四级沉淀，得到四种 PS 级分，分子量（MW）依次降低，PS40>PS50>PS70>PS80。四种 PS 级分均由四种单糖残基组成，甘露糖（Man）、葡萄糖（Glc）和半乳糖（Gal），但摩尔比不同。MW 最高（4.98×10）的 PS40 级分是最丰富的级分，占总 PS 质量的 64.4%，葡萄糖摩尔比也最高（~80%）。PS40 还能显著增强 RAW 264.7 细胞中 NO 和 ROS 的生成和吞噬活性。结果表明，AU 后进行分步乙醇沉淀是一种有效的策略，可减少溶剂消耗，从香菇中分离出主要的免疫刺激 PS。
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