Chakraborty J, Sinha Hikim A P, Jhunjhunwala J S
Exp Mol Pathol. 1986 Apr;44(2):222-9. doi: 10.1016/0014-4800(86)90072-9.
Unilateral torsion of the spermatic cord was surgically induced in 24 Hartley strain guinea pigs. Groups of six animals were sacrificed 4, 8, 12, and 16 months after the surgery and testes were excised. Nine animals has severely damaged testes. Extensive study on the Sertoli cells of each of these nine animals was carried out after testicular tissues were processed for electron microscopy following the usual procedure. The majority of the affected seminiferous tubules contained a single layer of Sertoli cells without any differentiating germ cells. The Sertoli cells were vacuolated and contained highly lobulated nuclei. Each nucleus contained a nucleolus. The Sertoli cells contained an elaborate annulate lamellae system. The membranous portion of the annulate lamellae was associated with the rough and smooth endoplasmic reticulum. Close association of the annulate lamellae with the lysosomes and lipid droplets was found to be a consistent feature. Although it was not possible for us to predict the functional significance of these annulate lamellae, the present investigation clearly established for the first time, the presence of an annulate lamellae system in the Sertoli cell of a rodent species.
在24只Hartley品系豚鼠中通过手术诱导造成精索单侧扭转。术后4、8、12和16个月分别处死每组6只动物并切除睾丸。9只动物的睾丸受到严重损伤。按照常规程序对这些动物的睾丸组织进行电子显微镜处理后，对其中9只动物的支持细胞进行了广泛研究。大多数受影响的生精小管含有单层支持细胞，没有任何分化的生殖细胞。支持细胞呈空泡状，细胞核高度分叶。每个细胞核都含有一个核仁。支持细胞含有一个复杂的环层板系统。环层板的膜部分与粗面内质网和滑面内质网相关。环层板与溶酶体和脂滴紧密相连是一个一致的特征。虽然我们无法预测这些环层板的功能意义，但本研究首次明确证实了啮齿动物支持细胞中存在环层板系统。
