Nistal M, Paniagua R
Z Mikrosk Anat Forsch. 1983;97(5):739-52.
Seminiferous tubules from infant testes show a mean tubular diameter of 70 micron with a high number of Sertoli cells (20...24 cells) per transverse tubular section which form a pseudostratified epithelium. Basal Sertoli cells have round to ovoid nuclei, regular in outline, with small nucleoli. The cytoplasm contains the usual organelles homogeneously distributed. Columnar Sertoli cells reach the lumen and show basal projections which surround the basal Sertoli cells. Basal regions of columnar cells contain numerous parallel microfilaments. Apical regions contain abundant rough endoplasmic reticulum, several Golgi complexes, and associated vesicles of electrondense content, Desmosome-like structures and extensive, flattened interdigitations are observed between Sertoli cells. Gap junctions between peritubular myofibroblasts and Sertoli cells are occasionally seen. During childhood the seminiferous tubules show a similar pattern, but the number of Sertoli cells per transverse tubular sections decreases (16...19 cells). From 9 years of age onwards the seminiferous tubules become thicker, the number of Sertoli cells decreases (12...15 cells), and the pseudostratified epithelium is gradually replaced by a columnar epithelium. The Sertoli cells begin to mature and smooth endoplasmic reticulum, mitochondria and lipid droplets develop. Annulate lamellae, crystals of Charcot-Böttcher, and Sertoli-Sertoli specialized junctions appear.
婴儿睾丸的生精小管平均管径为70微米，每个横切的生精小管切片中有大量支持细胞（20……24个细胞），这些细胞形成假复层上皮。基底支持细胞的细胞核呈圆形至卵圆形，轮廓规则，有小核仁。细胞质中含有均匀分布的常见细胞器。柱状支持细胞延伸至管腔，其基底突起围绕着基底支持细胞。柱状细胞的基部区域含有许多平行的微丝。顶端区域含有丰富的粗面内质网、几个高尔基体复合体以及相关的电子致密内容物小泡，在支持细胞之间观察到类似桥粒的结构和广泛的扁平指状交叉。偶尔可见管周肌成纤维细胞与支持细胞之间的缝隙连接。在儿童期，生精小管呈现类似的模式，但每个横切的生精小管切片中支持细胞的数量减少（16……19个细胞）。从9岁起，生精小管变粗，支持细胞数量减少（12……15个细胞），假复层上皮逐渐被柱状上皮取代。支持细胞开始成熟，滑面内质网、线粒体和脂滴开始发育。出现环状片层、夏科 - 博彻结晶以及支持细胞 - 支持细胞特化连接。
