谷胱甘肽二硫化物的能量关联心脏转运系统。

Energy-linked cardiac transport system for glutathione disulfide.

作者信息

Ishikawa T, Zimmer M, Sies H

出版信息

FEBS Lett. 1986 May 5;200(1):128-32. doi: 10.1016/0014-5793(86)80524-5.

Abstract

The relationship between the rate of glutathione disulfide (GSSG) export and the energy state was studied in isolated perfused rat heart. The intracellular GSSG level was maintained at saturation for transport (7.5 nmol GSSG X min-1 X g heart-1) by continuous perfusion with 20 microM t-butyl hydroperoxide. GSSG release was substantially restricted upon the addition of inhibitors of mitochondrial respiration such as KCN, antimycin A or rotenone. In contrast, no effect was observed on GSSG release during potassium-induced cardiac arrest, although changes in oxygen consumption and coronary flow were similar to those observed with KCN. The dependence of the GSSG transport rate on the cytosolic free ATP/ADP ratio reveals that GSSG transport is half-maximal at (ATP/ADP)free approximately equal to 10. The capacity of GSSG transport was unchanged by infusion of epinephrine, norepinephrine or dibutyryl cyclic AMP.

摘要

在离体灌注大鼠心脏中研究了谷胱甘肽二硫化物（GSSG）输出速率与能量状态之间的关系。通过用20μM叔丁基过氧化氢连续灌注，将细胞内GSSG水平维持在转运饱和状态（7.5 nmol GSSG×min-1×g心脏-1）。添加线粒体呼吸抑制剂如KCN、抗霉素A或鱼藤酮后，GSSG释放受到显著限制。相比之下，钾诱导的心脏停搏期间未观察到对GSSG释放的影响，尽管耗氧量和冠状动脉流量的变化与KCN观察到的相似。GSSG转运速率对胞质游离ATP/ADP比值的依赖性表明，GSSG转运在（ATP/ADP）游离约等于10时达到半最大。肾上腺素、去甲肾上腺素或二丁酰环磷酸腺苷的输注未改变GSSG转运能力。