二十年合作厘清噬菌体 Mu 的复制及其对 Mu 中心旋转酶结合位点的依赖性。

Twenty Years of Collaboration to Sort out Phage Mu Replication and Its Dependence on the Mu Central Gyrase Binding Site.

机构信息

Cellular and Molecular Microbiology, Université Libre de Bruxelles (ULB), Rue Adrienne Bolland 8 B, 6041 Gosselies, Belgium.

Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

出版信息

Viruses. 2023 Feb 27;15(3):637. doi: 10.3390/v15030637.

DOI:10.3390/v15030637

PMID:36992345

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10052514/

Abstract

For 20 years, the intricacies in bacteriophage Mu replication and its regulation were elucidated in collaboration between Ariane Toussaint and her co-workers in the Laboratory of Genetics at the Université Libre de Bruxelles, and the groups of Martin Pato and N. Patrick Higgins in the US. Here, to honor Martin Pato's scientific passion and rigor, we tell the history of this long-term sharing of results, ideas and experiments between the three groups, and Martin's final discovery of a very unexpected step in the initiation of Mu replication, the joining of Mu DNA ends separated by 38 kB with the assistance of the host DNA gyrase.

摘要

20 年来，Ariane Toussaint 与其在布鲁塞尔自由大学遗传学实验室的同事，以及美国 Martin Pato 和 N. Patrick Higgins 的团队合作，阐明了噬菌体 Mu 复制及其调控的复杂性。在这里，为了向 Martin Pato 的科学热情和严谨致敬，我们讲述了这三个小组之间长期分享成果、想法和实验的历史，以及 Martin 最终发现 Mu 复制起始过程中一个非常出人意料的步骤，即 Mu DNA 末端在宿主 DNA 回旋酶的协助下连接，相隔 38kB。