School of Public Health, Shanxi Medical University, 56 Xinjian South Road, Taiyuan 030001, China.
Taiyuan Center for Disease Control and Prevention, 89 Xinjian South Road, Taiyuan 030012, China.
Viruses. 2023 Mar 7;15(3):694. doi: 10.3390/v15030694.
In recent years, the prevalence of hand, foot, and mouth disease (HFMD) caused by enteroviruses other than enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) has gradually increased. The throat swab specimens of 2701 HFMD cases were tested, the regions of CVA10 RNA were amplified using RT-PCR, and phylogenetic analysis of CVA10 was performed. Children aged 1-5 years accounted for the majority (81.65%) and boys were more than girls. The positivity rates of EV-A71, CVA16, and other EVs were 15.22% (219/1439), 28.77% (414/1439), and 56.01% (806/1439), respectively. CVA10 is one of the important viruses of other EVs. A total of 52 CVA10 strains were used for phylogenetic analysis based on the region, 31 were from this study, and 21 were downloaded from GenBank. All CVA10 sequences could be assigned to seven genotypes (A, B, C, D, E, F, and G), and genotype C was further divided into C1 and C2 subtypes, only one belonged to subtype C1 and the remaining 30 belonged to C2 in this study. This study emphasized the importance of strengthening the surveillance of HFMD to understand the mechanisms of pathogen variation and evolution, and to provide a scientific basis for HFMD prevention, control, and vaccine development.
近年来，除肠道病毒 A71（EV-A71）和柯萨奇病毒 A16（CVA16）以外的其他肠道病毒引起的手足口病（HFMD）的流行率逐渐增高。采用 RT-PCR 扩增方法对 2701 例 HFMD 咽拭子标本进行检测，对 CVA10 RNA 区进行扩增，并对 CVA10 进行系统进化分析。患儿以 1-5 岁为主（81.65%），男多于女。EV-A71、CVA16 和其他 EV 的阳性率分别为 15.22%（219/1439）、28.77%（414/1439）和 56.01%（806/1439）。CVA10 是其他 EV 的重要病毒之一。基于 区共对 52 株 CVA10 进行了系统进化分析，其中 31 株为本研究分离株，21 株来源于 GenBank。所有 CVA10 序列可归为 7 个基因型（A、B、C、D、E、F 和 G），其中基因型 C 进一步分为 C1 和 C2 亚型，本研究仅发现 1 株属于 C1 亚型，其余 30 株属于 C2 亚型。本研究强调了加强 HFMD 监测的重要性，以了解病原体变异和进化的机制，为 HFMD 的预防、控制和疫苗开发提供科学依据。
