ICMR-National Institute of Virology, Bangalore, India.
ICMR-National Institute of Virology, Maximum Containment Facility, Microbial Containment Complex, Sus Road, Pashan, Pune 411021, India.
Infect Genet Evol. 2018 Dec;66:101-110. doi: 10.1016/j.meegid.2018.09.004. Epub 2018 Sep 12.
Non-Polio EnteroViruses (NPEV) are one of the known causative agents of Acute Flaccid Paralysis (AFP). In the present study, we identified, sequenced and characterized the complete genome of sixty-five Coxsackievirus-A10, an NPEV. These were isolated from stool specimens of AFP cases from Bihar, Karnataka, Kerala, and Uttar Pradesh (UP) states of India. Evolutionary analysis of complete genome (7420 nucleotides) and VP1 gene (894 nucleotides) demonstrates that there are four different intra-typic strains circulating in India which were dissimilar to Chinese strains. First intratypic strain circulating in UP, Bihar, and Karnataka; second in UP and Karnataka; third in UP and Bihar and; fourth was restricted only to Kerala state. The divergence of Kerala strain with respect to all other circulating strain of UP, Bihar and Karnataka states in India is 24%, 24.9%, and 24.4% respectively. Recombinations were observed between few of these strains which might be one of the factors of the observed intra-typic diversity. ARTICLE SUMMARY LINE: We report the identification, characterization and phylogenetic analysis of sixty-five Non-Polio Enterovirus (NPEV) isolates, performed during the year 2009-17, causing acute flaccid paralysis in pediatric cases with their divergences and recombinations from four states of India.
非脊髓灰质炎肠道病毒（NPEV）是急性弛缓性麻痹（AFP）的已知病原体之一。在本研究中，我们鉴定、测序并描述了 65 株柯萨奇病毒 A10 的完整基因组，这是一种 NPEV。这些病毒株从印度比哈尔邦、卡纳塔克邦、喀拉拉邦和北方邦（UP）的 AFP 病例的粪便标本中分离出来。对完整基因组（7420 个核苷酸）和 VP1 基因（894 个核苷酸）的进化分析表明，目前有四种不同的同种型病毒株在印度流行，它们与中国的病毒株不同。第一种在 UP、比哈尔邦和卡纳塔克邦流行；第二种在 UP 和卡纳塔克邦流行；第三种在 UP 和比哈尔邦流行；第四种仅局限于喀拉拉邦流行。与印度 UP、比哈尔邦和卡纳塔克邦流行的所有其他同种型病毒株相比，喀拉拉邦病毒株的差异分别为 24%、24.9%和 24.4%。这些病毒株之间观察到了重组，这可能是观察到的同种型多样性的因素之一。文章摘要：我们报告了在 2009 年至 2017 年期间，从印度四个邦的儿科 AFP 病例中分离出 65 株引起急性弛缓性麻痹的非脊髓灰质炎肠道病毒（NPEV），并对其进行了鉴定、特征描述和系统进化分析，及其与其他株之间的差异和重组。
