State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, China.
Front Cell Infect Microbiol. 2023 Mar 13;13:1109153. doi: 10.3389/fcimb.2023.1109153. eCollection 2023.
is a nosocomial bacterial pathogen that causes antibiotic-associated diarrhea mediated by cellular exotoxins secreted into the intestine during bacterial growth. Multilocus sequence typing (MLST) and PCR ribotyping are the main molecular typing for . Whole genome sequencing (WGS) core genome multilocus sequence typing (cgMLST) was developed for genetic evolution and outbreak investigation of with higher precision and accuracy.
A total of 699 whole (complete and draft) genome sequences of distinct strains were used in this study to identify core gene set (2469 core genes) and the cgMLST scheme for the phylogeny analysis of . This cgMLST pipeline was then carried the Chinese Pathogen Identification Net (China PIN) for surveillance of in China. Within the China PIN, 195 WGS of and an outbreak of CDI with 12 WGS of were used to evaluate the cgMLST pipeline.
The result displayed that mostly tested isolates could be successfully divided into 5 classic clades and the outbreak event was also successfully identified.
The results are meaningful and offer a practicable pipeline for a national-wide surveillance of in China.
艰难梭菌是一种医院获得性细菌病原体，它通过在细菌生长过程中分泌到肠道中的细胞外毒素引起抗生素相关性腹泻。多位点序列分型（MLST）和 PCR 核糖体分型是艰难梭菌的主要分子分型方法。全基因组测序（WGS）核心基因组多位点序列分型（cgMLST）用于艰难梭菌的遗传进化和暴发调查，具有更高的精度和准确性。
本研究共使用了 699 株不同的艰难梭菌全（完整和草图）基因组序列，用于鉴定核心基因集（2469 个核心基因）和 cgMLST 方案，以进行艰难梭菌的系统发育分析。然后，该 cgMLST 方案被用于中国病原体识别网络（China PIN）对中国艰难梭菌的监测。在中国 PIN 中，使用了 195 株艰难梭菌的 WGS 和 12 株艰难梭菌 CDI 暴发的 WGS，用于评估 cgMLST 方案。
结果显示，大多数测试的艰难梭菌分离株可以成功地分为 5 个经典分支，暴发事件也成功地得到了鉴定。
结果具有重要意义，为中国开展全国范围的艰难梭菌监测提供了可行的方案。
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