Abad-Fau Ana, Sevilla Eloísa, Martín-Burriel Inmaculada, Moreno Bernardino, Bolea Rosa
Departamento de Patología Animal, Facultad de Veterinaria, Instituto Agroalimentario de Aragon-IA2-(Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain.
Centro de Encefalopatías y Enfermedades Transmisibles Emergentes, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Microorganisms. 2023 Jul 5;11(7):1752. doi: 10.3390/microorganisms11071752.
This review aims to provide a comprehensive overview of the significant molecular typing techniques currently employed in research and medical communities. The main objectives of this review are to describe the key molecular typing methods utilized in studies and to highlight the epidemiological characteristics of the most prevalent strains on a global scale. Geographically distinct regions exhibit distinct strain types of , with notable concordance observed among various typing methodologies. The advantages that next-generation sequencing (NGS) offers has changed epidemiology research, enabling high-resolution genomic analyses of this pathogen. NGS platforms offer an unprecedented opportunity to explore the genetic intricacies and evolutionary trajectories of strains. It is relevant to acknowledge that novel routes of transmission are continually being unveiled and warrant further investigation, particularly in the context of zoonotic implications and environmental contamination.
本综述旨在全面概述目前研究和医学界所采用的重要分子分型技术。本综述的主要目的是描述研究中使用的关键分子分型方法,并突出全球范围内最流行菌株的流行病学特征。不同地理区域呈现出不同的菌株类型,各种分型方法之间存在显著的一致性。下一代测序(NGS)所提供的优势改变了流行病学研究,能够对这种病原体进行高分辨率的基因组分析。NGS平台为探索菌株的遗传复杂性和进化轨迹提供了前所未有的机会。需要认识到,新的传播途径不断被揭示,值得进一步研究,特别是在人畜共患病影响和环境污染方面。