Matters G L, Scandalios J G
Differentiation. 1986;30(3):190-6. doi: 10.1111/j.1432-0436.1986.tb00780.x.
Seeds of the inbred maize lines, W64A, R6-67, and D10, were germinated and grown at 25 degrees, 35 degrees, or 40 degrees C for up to 10 days. The catalase activity in scutella of W64A seedlings grown at 40 degrees C was slightly lower than that in seedlings grown at 25 degrees C. The total superoxide dismutase activity in scutella was lower in seedlings grown at 40 degrees C than in those grown at 25 degrees C during the first 3 days of germination, but thereafter was not significantly different at these temperatures. The high-catalase mutant lines, R6-67 and D10, grown at 40 degrees C exhibited a developmental pattern of catalase activity that was severalfold lower than that seen in seedlings grown at 25 degrees C. The decrease in catalase activity in R6-67 seedlings grown at 40 degrees C was correlated with lower amounts of CAT-2 protein, which is normally present at significantly high levels in this line. The application of a catalase synthesis inhibitor revealed that the low levels of CAT-2 in R6-67 grown at 40 degrees C were due to slightly higher degradation rates and a significant drop in the rate of catalase protein synthesis.
自交系玉米W64A、R6 - 67和D10的种子在25℃、35℃或40℃下发芽并生长长达10天。在40℃下生长的W64A幼苗盾片中的过氧化氢酶活性略低于在25℃下生长的幼苗。在发芽的前3天，40℃下生长的幼苗盾片中的总超氧化物歧化酶活性低于25℃下生长的幼苗，但此后在这些温度下没有显著差异。在40℃下生长的高过氧化氢酶突变系R6 - 67和D10表现出的过氧化氢酶活性发育模式比在25℃下生长的幼苗低几倍。在40℃下生长的R6 - 67幼苗中过氧化氢酶活性的降低与CAT - 2蛋白含量较低有关，该蛋白在该品系中通常以显著高水平存在。过氧化氢酶合成抑制剂的应用表明，在40℃下生长的R6 - 67中CAT - 2水平较低是由于降解速率略高以及过氧化氢酶蛋白合成速率显著下降。
