King Elise R, Garrett Haley E, Abernathy Haley, Cassidy Caitlin A, Cabell Cameron R, Shook-Sa Bonnie E, Juliano Jonathan J, Boyce Ross M, Aiello Allison E, Ciccone Emily J
medRxiv. 2023 Mar 20:2023.03.13.23286935. doi: 10.1101/2023.03.13.23286935.
Measuring seroprevalence over time is a valuable epidemiological tool for improving our understanding of COVID-19 immunity. Due to the large number of collections required for population surveillance as well as concerns about potential infection risk to the collectors, self-collection approaches are being increasingly pursued. To advance this methodology, we collected paired venous and capillary blood samples by routine phlebotomy and Tasso-SST device respectively from 26 participants and measured total immunoglobulin (Ig) and IgG antibodies to the SARS-CoV-2 receptor binding domain (RBD) by enzyme-linked immunosorbent assay (ELISA) on both specimens. Qualitatively, no discrepancies were noted in binary results between Tasso and venipuncture-derived plasma. Furthermore, in vaccinated participants, correlation between Tasso and venous total Ig and IgG specific antibody quantitative levels was high (Total Ig: ρ = 0.72, 95% CI (0.39- 0.90); IgG: ρ = 0.85, 95% CI (0.54, 0.96)). Our results support the use of Tasso at-home collection devices for antibody testing.
长期测量血清阳性率是一种有价值的流行病学工具，有助于增进我们对新冠病毒免疫力的了解。由于人群监测需要大量样本采集，同时担心采集人员存在潜在感染风险，自我采集方法正越来越受到关注。为了推进这种方法，我们分别通过常规静脉穿刺和Tasso-SST设备从26名参与者中采集了配对的静脉血和毛细血管血样本，并通过酶联免疫吸附测定（ELISA）对两个样本检测了针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）受体结合域（RBD）的总免疫球蛋白（Ig）和IgG抗体。定性分析表明，Tasso检测结果与静脉穿刺血浆的二元结果之间没有差异。此外，在接种疫苗的参与者中，Tasso检测结果与静脉血总Ig和IgG特异性抗体定量水平之间的相关性很高（总Ig：ρ = 0.72，95%可信区间（0.39 - 0.90）；IgG：ρ = 0.85，95%可信区间（0.54，0.96））。我们的结果支持使用Tasso家庭采集设备进行抗体检测。
