Zaree Pouya, Tomris Ilhan, de Vos Sander D, van der Woude Roosmarijn, Flesch Frits M, Klein Gebbink Robertus J M, de Vries Robert P, Pieters Roland J
Department of Chemical Biology & Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University P. O. Box 80082 NL-3508 TB Utrecht The Netherlands
Institute for Sustainable and Circular Chemistry, Debye Institute for Nanomaterials Science, Utrecht University Universiteitsweg 99 3584 CG Utrecht The Netherlands.
RSC Adv. 2023 Mar 27;13(14):9756-9760. doi: 10.1039/d3ra01029e. eCollection 2023 Mar 20.
A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.
一种用于电化学检测的新型微型传感器，其包含石墨烯和金纳米颗粒，并通过蛋白质进行了功能化处理。使用循环伏安法（CV）和差分脉冲伏安法（DPV），可以观察和量化分子与这些蛋白质的相互作用。蛋白质结合物包括小至碳水化合物的碳水化合物配体，直至参与蛋白质-蛋白质相互作用的新冠病毒刺突蛋白变体。该系统使用现成的传感器与价格实惠的恒电位仪相结合，并且对小分子配体结合足够灵敏。
