基于转录组测序和基因分析的电针对足三里（ST36）跳跃损伤肌肉的疗效。

Efficacy of electroacupunture at Zusanli (ST36) on jumping-injured muscle based on transcriptome sequencing and genes analysis.

机构信息

School of Physical Education and Sport, Henan University, Kaifeng 475001, China.

Kidney Department, Xindu Hospital of Traditional Chinese Medicine, Chengdu 610500, China.

出版信息

J Tradit Chin Med. 2023 Apr;43(2):322-328. doi: 10.19852/j.cnki.jtcm.20221121.001.

DOI:10.19852/j.cnki.jtcm.20221121.001

PMID:36994520

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10012191/

Abstract

OBJECTIVE

To reveal the efficacy and possible mechanism of electroacupuncture at Zusanli (ST36) on injured skeletal muscle induced by jumping load.

METHODS

In the present study, female Sprague-Dawley rats were randomly divided into four groups with 6 of each, including normal control group (NC), jumping-induced muscle injury model group (JI), JI with electroacupuncture stimulation treatment group (EA), and JI with non-electroacupuncture stimulation group (NEA). Transmission electron microscopy, transcriptome sequencing and analysis, prediction of protein interaction networks, real-time polymerase chain reaction verification, and Western blotting were performed on the gastrocnemius muscle of ipsilateral lower limbs.

RESULTS

The structural repair of injured gastrocnemius myofibers following jumping training in EA rats was better than that of NEA rats. A total of 136 genes were differentially expressed in EA rats relative to JI rats, with 55 genes upregulated and 81 genes downregulated. According to results of transcriptome analysis, and prediction of protein mutual interaction by the online STRING database, Heat shock protein beta-7 (Hspb7) and myozenin2 (Myoz2) genes were targeted. Expressions of Hspb7 and Myoz2 mRNAs were increased in EA rats relative to JI rats (0.05). The expression of Hspb7 protein was upregulated in EA rats relative to that in NC, JI, and NEA rats (0.01, 0.05, and0.05, respectively). The expression of Myoz2 protein was upregulated in EA rats relative to that in NC and JI rats (both 0.01respectively).

CONCLUSIONS

The present results suggest that electroacupuncture stimulation at Zusanli (ST36) could improve muscle healing following jumping-induced muscle injury, owing to the upregulation of Hspb7 and Myoz2 proteins.

摘要

目的

揭示电针对跳跃负荷致损伤骨骼肌的疗效及可能机制。

方法

本研究将雌性 Sprague-Dawley 大鼠随机分为 4 组，每组 6 只，包括正常对照组（NC）、跳跃致肌肉损伤模型组（JI）、电针刺激治疗组（EA）和非电针刺激组（NEA）。对同侧下肢腓肠肌进行透射电镜、转录组测序及分析、蛋白互作网络预测、实时聚合酶链反应验证和 Western blot 检测。

结果

EA 大鼠跳跃训练后损伤腓肠肌肌纤维结构修复优于 NEA 大鼠。与 JI 大鼠相比，EA 大鼠有 136 个基因差异表达，其中 55 个基因上调，81 个基因下调。根据转录组分析结果和在线 STRING 数据库预测的蛋白互作，选择热休克蛋白β-7（Hspb7）和肌联蛋白 2（Myoz2）基因。EA 大鼠 Hspb7 和 Myoz2mRNA 的表达均高于 JI 大鼠（0.05）。EA 大鼠 Hspb7 蛋白的表达高于 NC、JI 和 NEA 大鼠（均为 0.01）。EA 大鼠 Myoz2 蛋白的表达高于 NC 和 JI 大鼠（均为 0.01）。