Lou Linjiang, Frishman Laura J, Beach Krista M, Rajagopalan Lakshmi, Hung Li-Fang, She Zhihui, Smith Earl L, Ostrin Lisa A
College of Optometry, University of Houston, Houston, TX, USA.
Brien Holden Vision Institute, Sydney, NSW, Australia.
Doc Ophthalmol. 2023 Aug;147(1):45-57. doi: 10.1007/s10633-023-09931-0. Epub 2023 Mar 30.
Exposure to blue light is thought to be harmful to the retina. The purpose of this study was to determine the effects of long-term exposure to narrowband blue light on retinal function in rhesus monkeys.
Young rhesus monkeys were reared under short-wavelength "blue" light (n = 7; 465 nm, 183 ± 28 lx) on a 12-h light/dark cycle starting at 26 ± 2 days of age. Age-matched control monkeys were reared under broadband "white" light (n = 8; 504 ± 168 lx). Light- and dark-adapted full-field flash electroretinograms (ERGs) were recorded at 330 ± 9 days of age. Photopic stimuli were brief red flashes (0.044-5.68 cd.s/m) on a rod-saturating blue background and the International Society for Clinical Electrophysiology of Vision (ISCEV) standard 3.0 white flash on a 30 cd/m white background. Monkeys were dark adapted for 20 min and scotopic stimuli were ISCEV standard white flashes of 0.01, 3.0, and 10 cd.s/m. A-wave, b-wave, and photopic negative response (PhNR) amplitudes were measured. Light-adapted ERGs in young monkeys were compared to ERGs in adult monkeys reared in white light (n = 10; 4.91 ± 0.88 years of age).
For red flashes on a blue background, there were no significant differences in a-wave (P = 0.46), b-wave (P = 0.75), and PhNR amplitudes (P = 0.94) between white light and blue light reared monkeys for all stimulus energies. ISCEV standard light- and dark-adapted a- and b-wave amplitudes were not significantly different between groups (P > 0.05 for all). There were no significant differences in a- and b-wave implicit times between groups for all ISCEV standard stimuli (P > 0.05 for all). PhNR amplitudes of young monkeys were significantly smaller compared to adult monkeys for all stimulus energies (P < 0.05 for all). There were no significant differences in a-wave (P = 0.19) and b-wave (P = 0.17) amplitudes between young and adult white light reared monkeys.
Long-term exposure to narrowband blue light did not affect photopic or scotopic ERG responses in young monkeys. Findings suggest that exposure to 12 h of daily blue light for approximately 10 months does not result in altered retinal function.
蓝光照射被认为对视网膜有害。本研究的目的是确定长期暴露于窄带蓝光对恒河猴视网膜功能的影响。
幼年恒河猴在短波长“蓝光”（n = 7；465纳米，183±28勒克斯）下饲养，光照/黑暗周期为12小时，从26±2日龄开始。年龄匹配的对照猴在宽带“白光”（n = 8；504±168勒克斯）下饲养。在330±9日龄时记录明适应和暗适应的全视野闪光视网膜电图（ERG）。明视刺激是在杆饱和蓝光背景上的短暂红色闪光（0.044 - 5.68坎德拉·秒/平方米）以及在30坎德拉/平方米白色背景上的国际临床视觉电生理学会（ISCEV）标准3.0白色闪光。猴子暗适应20分钟，暗视刺激是ISCEV标准的0.01、3.0和10坎德拉·秒/平方米的白色闪光。测量A波、B波和明视负反应（PhNR）的振幅。将幼年猴子的明适应ERG与在白光下饲养的成年猴子（n = 10；4.91±0.88岁）的ERG进行比较。
对于蓝光背景上的红色闪光，在所有刺激能量下，白光饲养和蓝光饲养的猴子之间，A波（P = 0.46）、B波（P = 0.75）和PhNR振幅（P = 0.94）没有显著差异。ISCEV标准的明适应和暗适应A波和B波振幅在两组之间没有显著差异（所有P > 0.05）。对于所有ISCEV标准刺激，两组之间A波和B波的隐含时间没有显著差异（所有P > 0.05）。对于所有刺激能量，幼年猴子的PhNR振幅与成年猴子相比显著较小（所有P < 0.05）。幼年和成年白光饲养的猴子之间，A波（P = 0.19）和B波（P = 0.17）振幅没有显著差异。
长期暴露于窄带蓝光不会影响幼年猴子的明视或暗视ERG反应。研究结果表明，每天暴露于蓝光12小时，持续约10个月不会导致视网膜功能改变。
