Kayani Farrukh Bashir, Rafique Saima, Akram Rizwan, Hussain Mozaffar, Bashir Shazia, Nasir Rubina, Khan Jan Sher
Department of Physics, Air University, PAF Complex, E-9, Islamabad 44000, Pakistan.
Department of Physics & Applied Mathematics, Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, 45650, Pakistan.
Nanotechnology. 2023 Apr 13;34(26). doi: 10.1088/1361-6528/acc8d8.
Metal oxide-based sensors have the benefit of inexpensive, quick response, and high sensitivity in detecting specific biological species. In this article, a simple electrochemical immunosensor was fabricated using antibody-chitosan coated silver/cerium oxide (Ab-CS@Ag/CeO) nanocomposites on a gold electrode for sensitive alpha-fetoprotein (AFP) diagnosis in human serum samples. Successfully synthesis of AFP antibody-CS@Ag/CeOconjugates was confirmed through Fourier transform infrared spectra of the prototype. The amine coupling bond chemistry was then used to immobilize the resultant conjugate on a gold electrode surface. It was observed that the interaction of the synthesized Ab-CS@Ag/CeOnanocomposites with AFP prevented an electron transfer and reduced the voltammetric Fe(CN)peak current, which was proportional to the amount of AFP. The linear ranges of AFP concentration were found from 10-10g.ml. The limit of detection was calculated using the calibration curve and came out to be 0.57 pg.ml. The designed label-free immunosensor successfully detected AFP in human serum samples. As a result, the resulting immunosensor is a promising sensor plate form for AFP detection and could be used in clinical bioanalysis.
基于金属氧化物的传感器在检测特定生物物种方面具有成本低、响应快和灵敏度高的优点。在本文中，一种简单的电化学免疫传感器被构建，该传感器在金电极上使用抗体-壳聚糖包被的银/氧化铈（Ab-CS@Ag/CeO）纳米复合材料，用于灵敏诊断人血清样本中的甲胎蛋白（AFP）。通过原型的傅里叶变换红外光谱证实了AFP抗体-CS@Ag/CeO缀合物的成功合成。然后利用胺偶联键化学将所得缀合物固定在金电极表面。观察到合成的Ab-CS@Ag/CeO纳米复合材料与AFP的相互作用阻止了电子转移并降低了伏安法Fe(CN)峰电流，该电流与AFP的量成比例。AFP浓度的线性范围为10 - 10g.ml。使用校准曲线计算检测限，结果为0.57 pg.ml。所设计的无标记免疫传感器成功检测了人血清样本中的AFP。因此，所得免疫传感器是一种用于AFP检测的有前景的传感器平台，可用于临床生物分析。
