中国儿童中肠道微生物群与肥胖合并颈动脉内膜中层厚度增厚的关系。

Association between gut microbiota and obesity combined with high carotid intima-media thickness among Chinese children.

机构信息

Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Email:

出版信息

Asia Pac J Clin Nutr. 2023;32(1):93-105. doi: 10.6133/apjcn.202303_32(1).0014.

DOI:10.6133/apjcn.202303_32(1).0014

PMID:36997491

Abstract

BACKGROUND AND OBJECTIVES

Obesity and related target organ damage such as high carotid intima-media thickness (cIMT) in children is associated with cardiovascular disease (CVD) later in life. However, the asso-ciation between gut microbiota and obesity combined with high cIMT among children remains unclear. Therefore, we compared differences in composition, community diversity, and richness of gut microbiota among normal children and obesity combined with or without high cIMT to identify differential microbiota biomarkers.

METHODS AND STUDY DESIGN

A total of 24 children with obesity combined with high cIMT (OB+high-cIMT), 24 with obesity but normal cIMT (OB+non-high cIMT), and 24 with normal weight and normal cIMT aged 10-11 years matched by age and sex from the "Huantai Childhood Cardiovascular Health Cohort Study" were included. All included fecal samples were tested using 16S rRNA gene sequencing.

RESULTS

The community richness and diversity of gut microbiota in OB+high-cIMT children were decreased compared with OB+non-high cIMT children and normal children. At the genus level, the relative abundances of Christensenellaceae_R-7_group, UBA1819, Family_XIII_AD3011_group, and unclassi-fied_o_Bacteroidales were associated with reduced odds of OB+high-cIMT among children. Receiver operating characteristic (ROC) analysis showed that combined Christensenellaceae_R-7_group, UBA1819, Fami-ly_XIII_AD3011_group, and unclassified_o_Bacteroidales performed a high ability in identifying OB+high-cIMT. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that several pathways such as biosynthesis of amino acids and aminoacyl-tRNA pathways were lower in the OB+high-cIMT group compared with the normal group.

CONCLUSIONS

We found that the alteration of gut microbiota was associated with OB+high-cIMT among children, which indicates that the gut microbiota may be a marker for obesity and related cardiovascular damage among children.

摘要

背景和目的

肥胖症和相关的靶器官损伤，如儿童的颈动脉内膜中层厚度（cIMT）升高，与日后的心血管疾病（CVD）有关。然而，儿童的肠道微生物群与肥胖症以及与 cIMT 升高的关系仍不清楚。因此，我们比较了正常儿童、肥胖症且 cIMT 正常（OB+non-high cIMT）和肥胖症且 cIMT 升高（OB+high-cIMT）儿童之间肠道微生物群的组成、群落多样性和丰富度的差异，以确定差异微生物群生物标志物。

方法和研究设计

本研究共纳入 24 名肥胖症且 cIMT 升高的儿童（OB+high-cIMT）、24 名肥胖症且 cIMT 正常的儿童（OB+non-high cIMT）和 24 名年龄和性别匹配的正常体重且 cIMT 正常的儿童，所有纳入的粪便样本均采用 16S rRNA 基因测序进行检测。

结果

与 OB+non-high cIMT 儿童和正常儿童相比，OB+high-cIMT 儿童的肠道微生物群群落丰富度和多样性降低。在属水平上，Christensenellaceae_R-7_group、UBA1819、Family_XIII_AD3011_group 和未分类的 o_Bacteroidales 的相对丰度与儿童中 OB+high-cIMT 的可能性降低相关。接收者操作特征（ROC）分析表明，Christensenellaceae_R-7_group、UBA1819、Family_XIII_AD3011_group 和未分类的 o_Bacteroidales 的组合在识别 OB+high-cIMT 方面具有较高的能力。未观察到状态的群落重建系统发育分析（PICRUSt）显示，与正常组相比，OB+high-cIMT 组的几种途径，如氨基酸生物合成和氨酰-tRNA 途径较低。