出生体重与健康婴儿肥胖和糖尿病基因表达的关系：一项病例对照研究。
Birth weight concerning obesity and diabetes gene expression in healthy infants; a case-control study.
机构信息
Molecular and Experimental Nutritional Medicine Department, University of Potsdam, Nuthetal, Germany.
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
出版信息
BMC Pregnancy Childbirth. 2023 Mar 30;23(1):218. doi: 10.1186/s12884-023-05538-0.
BACKGROUND
Since obesity and diabetes are prevalent worldwide, identifying the factors affecting these two conditions can effectively alter them. We decided to investigate the expression of obesity and diabetes genes in infants with birth weights lower than 2500 g in comparison with infants with normal birth weights.
METHODS
215 healthy infants between the ages of 5-6 months were used in the current case-control research, which was conducted at health and treatment facilities in Kermanshah. Infants who were healthy were chosen for the research after their weight and height were measured and compared to the WHO diagram to ensure that they were well-grown and in good health. There were 137 infants in the control group and 78 infants in the case group. All newborns had 5 cc of blood drawn intravenously. To assess the expression of the genes MC4R, MTNR1B, PTEN, ACACB, PPAR-γ, PPAR-α, NRXN3, NTRK2, PCSK1, A2BP1, TMEM18, LXR, BDNF, TCF7L2, FTO and CPT1A, blood samples were gathered in EDTA-coated vials. Chi-square, Mann-Whitney U, and Spearman analyses were used to examine the data.
RESULTS
A significant inverse correlation between birth weight and obesity and diabetes genes, including MTNR1B, NTRK2, PCSK1, and PTEN genes (r= -0.221, -0.235, -0.246, and - 0.418, respectively). In addition, the LBW infant's expression level was significantly up-regulated than the normal-weight infants (P = 0.001, 0.007, 0.001, and < 0.001, respectively). The expression level of the PPAR-a gene had a significantly positive correlation with birth weight (r = 0.19, P = 0.005). The expression level of the PPAR-a gene in the normal-weight infants was significantly up-regulated than the LBW infants (P = 0.049).
CONCLUSION
The expression levels of MTNR1B, NTRK2, PCSK1, and PTEN genes were up-regulated in the LBW infants; however, the expression level of PPAR-a gene was significantly down-regulated in the LBW infants compared to the infants with normal birth weight.
背景
由于肥胖和糖尿病在全球范围内普遍存在，因此确定影响这两种疾病的因素可以有效地改变它们。我们决定研究出生体重低于 2500 克的婴儿与出生体重正常的婴儿相比，肥胖和糖尿病基因的表达情况。
方法
本病例对照研究在克尔曼沙阿的卫生和治疗机构进行，共纳入 215 名 5-6 个月龄的健康婴儿。在测量并比较世卫组织图表后，选择体重和身高均符合标准、生长良好且健康的婴儿作为研究对象。对照组有 137 名婴儿，病例组有 78 名婴儿。所有新生儿均经静脉抽取 5cc 血液。为评估 MC4R、MTNR1B、PTEN、ACACB、PPAR-γ、PPAR-α、NRXN3、NTRK2、PCSK1、A2BP1、TMEM18、LXR、BDNF、TCF7L2、FTO 和 CPT1A 等基因的表达情况，采集 EDTA 抗凝管中的血样。采用卡方检验、Mann-Whitney U 检验和 Spearman 分析对数据进行检验。
结果
出生体重与肥胖和糖尿病基因（包括 MTNR1B、NTRK2、PCSK1 和 PTEN 基因）呈显著负相关（r 值分别为-0.221、-0.235、-0.246 和-0.418）。此外，LBW 婴儿的基因表达水平显著高于正常体重婴儿（分别为 P=0.001、0.007、0.001 和<0.001）。PPAR-α 基因的表达水平与出生体重呈显著正相关（r=0.19，P=0.005）。与 LBW 婴儿相比，正常体重婴儿的 PPAR-α 基因表达水平显著上调（P=0.049）。
结论
与出生体重正常的婴儿相比，LBW 婴儿的 MTNR1B、NTRK2、PCSK1 和 PTEN 基因表达水平上调，而 PPAR-α 基因表达水平下调。
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