Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon, Republic of Korea; Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, Republic of Korea.
Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon, Republic of Korea.
Stem Cell Res. 2023 Jun;69:103081. doi: 10.1016/j.scr.2023.103081. Epub 2023 Mar 23.
PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-β), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-β plays an essential role in early liver diseases, including liver fibrosis. Here, we generated a human induced pluripotent stem cell (iPSC) line, KITi001-A-1, using CRISPR/Cas9. This reporter iPSC line and its derivatives are useful for tracing PDGFR-β-expressing cells and for screening for liver fibrosis-inducing compounds.
PDGFRB 编码血小板衍生生长因子受体 β（PDGFR-β），是血小板衍生生长因子家族成员的细胞表面酪氨酸激酶受体。它是血管和神经系统正常发育以及肌动蛋白细胞骨架重排所必需的。PDGFR-β 在包括肝纤维化在内的早期肝病中发挥着重要作用。在这里，我们使用 CRISPR/Cas9 生成了一个人类诱导多能干细胞（iPSC）系，KITi001-A-1。该报告基因 iPSC 系及其衍生物可用于追踪表达 PDGFR-β 的细胞，并筛选诱导肝纤维化的化合物。
