Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon, Republic of Korea; Laboratory of Stem Cells and Tissue Regeneration, Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, Republic of Korea.
Department of Predictive Toxicology, Korea Institute of Toxicology, Daejeon, Republic of Korea.
Stem Cell Res. 2021 Oct;56:102524. doi: 10.1016/j.scr.2021.102524. Epub 2021 Aug 30.
Alpha-smooth muscle actin (α-SMA) is encoded by ACTA2 and is a key protein in the cellular contractile system of various mesodermal cell types, including hepatic stellate cells (HSCs), smooth muscle cells, and cardiomyocytes. α-SMA, which is a key protein in the development of hepatic fibrosis, is widely used as a reliable marker of HSC activation. Here, we generated an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using a CRISPR/Cas9-based knock-in system. These reporter hiPSC lines can be used for lineage tracing of mesodermal cells and for screening of HSC activation factors.
α-平滑肌肌动蛋白（α-SMA）由 ACTA2 编码，是各种中胚层细胞类型（包括肝星状细胞（HSCs）、平滑肌细胞和心肌细胞）细胞收缩系统的关键蛋白。α-SMA 是肝纤维化发展的关键蛋白，被广泛用作 HSC 激活的可靠标志物。在这里，我们使用基于 CRISPR/Cas9 的基因敲入系统生成了 ACTA2-EGFP 报告人人类诱导多能干细胞系 KITi001-C-41。这些报告人 hiPSC 系可用于中胚层细胞的谱系追踪和 HSC 激活因子的筛选。
