Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Material Science, South-central University of Nationalities, Wuhan 430074, China.
Analyst. 2016 Sep 21;141(18):5450-5. doi: 10.1039/c6an00905k. Epub 2016 Jun 29.
Using 6-hydroxyl-quinoline-2-benzothiazole (HQB) as a two-photon fluorophore and tert-butyldiphenylsilyl as a recognition domain for F(-), a ratiometric two-photon fluorescent fluoride probe, QF, was synthesized and fully characterized. QF displays both one- and two-photon ratiometric responses towards fluoride ions in aqueous solution. QF was enabled to detect exogenous fluoride ions in living cells by a ratiometric method. Two-photon microscopic imaging of fluoride ions in living HeLa cells and zebrafish has also been achieved. QF has been demonstrated to be an excellent fluorescent probe with high selectivity, low cytotoxicity and good photostability.
使用 6-羟基喹啉-2-苯并噻唑（HQB）作为双光子荧光团，叔丁基二苯基硅烷作为 F(-)的识别域，合成并充分表征了比率型双光子荧光氟化物探针 QF。QF 在水溶液中对氟离子表现出单光子和双光子比率响应。QF 通过比率法实现了对活细胞中外源氟离子的检测。还实现了活 HeLa 细胞和斑马鱼中氟离子的双光子显微镜成像。QF 被证明是一种具有高选择性、低细胞毒性和良好光稳定性的优秀荧光探针。
