Zhang Pan, Xue Ke, Dai Yanpeng, Zhao Xinxin, Zhang Dongdong, Wei Pengfei, Qi Zhengjian
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, PR China.
Special Equipment Safety Supervision Inspection Institue of Jiangsu Province, Nanjing, Jiangsu 210003, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5;296:122657. doi: 10.1016/j.saa.2023.122657. Epub 2023 Mar 21.
High-fidelity imaging and long-term visualization of lysosomes are pivotal factors in the functional assessment of lysosomes, which perform an instrumental role in the physiological activity of cells. However, commercial probes have great limitations in lysosome exploration resulting from the aggregation-caused quenching effect as well as photobleaching instability and small Stokes shift. Therefore, we constructed a novel probe named TTAM with triphenylamine as the matrix and morpholine ring as the targeting group. In contrast with commonly accessible Lyso-tracker Red, TTAM has the merits of aggregation-induced emission effect, extremely high quantum yields (51.57 % solid-state) as well as fluorescence intensity, significant photostability, and high resolution. These properties make it ideal for imaging and activity monitoring lysosomes, which provides a powerful condition for bio-imaging.
溶酶体的高保真成像和长期可视化是溶酶体功能评估的关键因素，溶酶体在细胞的生理活动中发挥着重要作用。然而，由于聚集诱导猝灭效应、光漂白不稳定性和斯托克斯位移小等问题，商业探针在溶酶体探索方面存在很大局限性。因此，我们构建了一种新型探针TTAM，以三苯胺为基质，吗啉环为靶向基团。与常用的溶酶体示踪剂Lyso-tracker Red相比，TTAM具有聚集诱导发光效应、极高的量子产率（固态为51.57%）以及荧光强度、显著的光稳定性和高分辨率等优点。这些特性使其成为溶酶体成像和活性监测的理想选择，为生物成像提供了有力条件。
