亚细胞膜-抗体结合测定法在人类肿瘤抗原表达分析中的应用。
Application of a subcellular membrane-antibody binding assay for the analysis of antigen expression in human tumours.
作者信息
Price M R, Edwards S, Baldwin R W
出版信息
J Cancer Res Clin Oncol. 1986;111(2):169-72. doi: 10.1007/BF00400759.
Abstract
A micro-radioisotopic antiglobulin assay has been employed to determine the binding of monoclonal antibodies to subcellular membranes adsorbed to the wells of Terasaki Microtest Plates. A panel of 15 monoclonal antibodies was tested against 22 membrane preparations isolated from normal and malignant colo-rectal, breast and lung tissues. The assay provided an objective evaluation of antibody reactivity permitting the expression of major antigens in samples from individual surgical specimens to be defined.
摘要
一种微量放射性同位素抗球蛋白测定法已被用于确定单克隆抗体与吸附在特拉萨基微量试验板孔中的亚细胞膜的结合。使用一组15种单克隆抗体对从正常和恶性结肠、直肠、乳腺和肺组织中分离出的22种膜制剂进行了测试。该测定法对抗体反应性进行了客观评估，从而能够确定来自各个手术标本的样本中主要抗原的表达情况。
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