University of Passo Fundo, Campus I, km 171, BR 285, P. O. Box 611, Passo Fundo City, Rio Grande do Sul State, 99001-970, Brazil.
Federal University of the South Border - Campus Erechim, RS 135, Km 72, Erechim City, Rio Grande do Sul State, 99700-000, Brazil.
World J Microbiol Biotechnol. 2023 Apr 1;39(6):144. doi: 10.1007/s11274-023-03588-2.
The commercialization of fruits in markets generates a large amount of waste because they are perishable and have a short shelf life, so, they are discarded. This study aimed to provide a noble end to discarded fruits that have fermentable sugars. Banana, apple, mango and papaya residues were collected from supermarkets and underwent an enzymatic hydrolysis process. The ability of four pectinases, two amylases, one xylanase and one cellulase to release reducing sugars from fruit biomass before fermentation with two yeast strains (S. cerevisiae CAT-1 and S. cerevisiae Angel) for bioethanol production was investigated, obtaining a total of RS (Reducing sugar) of 268.08 mg/mL in banana residues. A fermentation with yeast S. cerevisiae CAT-1 resulted in 98% consumption of RS and the production of a total of 28.02 g/L of ethanol. Furthermore, fermentation with the yeast S. cerevisiae Angel, resulted in 97% RS consumption and 31.87 g/L ethanol production, which was the best result obtained throughout all the tests of hydrolysis, highlighting the banana residue as a promising biomass for the production of bioethanol.
水果在市场上的商业化会产生大量的浪费，因为它们容易腐烂，保质期短，所以通常会被丢弃。本研究旨在为具有可发酵糖的废弃水果提供一个有价值的归宿。从超市收集了香蕉、苹果、芒果和木瓜的残余物，并进行了酶水解处理。考察了四种果胶酶、两种淀粉酶、一种木聚糖酶和一种纤维素酶在与两种酵母菌株（S. cerevisiae CAT-1 和 S. cerevisiae Angel）发酵前从水果生物质中释放还原糖的能力，用于生物乙醇生产，从香蕉残余物中获得了 268.08 mg/mL 的总还原糖（RS）。酵母 S. cerevisiae CAT-1 的发酵导致 98%的 RS 被消耗，总共产生了 28.02 g/L 的乙醇。此外，酵母 S. cerevisiae Angel 的发酵导致 97%的 RS 被消耗，产生了 31.87 g/L 的乙醇，这是在所有水解试验中获得的最佳结果，突出了香蕉残余物作为生产生物乙醇的有前途的生物质。
