State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
Sci Total Environ. 2023 Jul 10;881:163218. doi: 10.1016/j.scitotenv.2023.163218. Epub 2023 Mar 31.
Antibiotics and disinfectants have both been proposed to exert selective pressures on the biofilm as well as affecting the emergence and spread of antibiotic resistance genes (ARGs). However, the transfer mechanism of ARGs in drinking water distribution system (DWDS) under the coupling effect of antibiotics and disinfectants has not been completely understood. In the current study, four lab-scale biological annular reactors (BARs) were constructed to evaluate the effects of sulfamethoxazole (SMX) and NaClO coupling in DWDS and reveal the related mechanisms of ARGs proliferation. TetM was abundant in both the liquid phase and the biofilm, and redundancy analysis showed that the total organic carbon (TOC) and temperature were significantly correlated with ARGs in the water phase. There was a significant correlation between the relative abundance of ARGs in the biofilm phase and extracellular polymeric substances (EPS). Additionally, the proliferation and spread of ARGs in water phase were related to microbial community structure. Partial least-squares path modeling showed that antibiotic concentration may influence ARGs by affecting MGEs. These findings help us to better understand the diffusion process of ARGs in drinking water and provide a theoretical support for technologies to control ARGs at the front of pipeline.
抗生素和消毒剂都被提议对生物膜施加选择性压力，同时影响抗生素耐药基因（ARGs）的出现和传播。然而，在抗生素和消毒剂耦合作用下，饮用水分配系统（DWDS）中 ARGs 的转移机制尚未完全了解。在本研究中，构建了四个实验室规模的生物环形反应器（BARs），以评估磺胺甲恶唑（SMX）和 NaClO 在 DWDS 中的耦合作用的影响，并揭示 ARGs 增殖的相关机制。液体相和生物膜中均存在大量 TetM，冗余分析表明，TOC 和温度与水相中 ARGs 显著相关。生物膜相中 ARGs 的相对丰度与胞外聚合物（EPS）之间存在显著相关性。此外，水相中的 ARGs 的增殖和传播与微生物群落结构有关。偏最小二乘路径模型表明，抗生素浓度可能通过影响 MGEs 来影响 ARGs。这些发现有助于我们更好地了解饮用水中 ARGs 的扩散过程，并为控制管道前端 ARGs 的技术提供理论支持。
