State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China.
Environ Pollut. 2023 Oct 15;335:122311. doi: 10.1016/j.envpol.2023.122311. Epub 2023 Aug 3.
Due to the intensive use of antibiotics, the drinking water distribution system (DWDS) has become one of the hotspots of antibiotic resistance. However, little is known about the role of biofilm in the aspect of spreading resistance in DWDS. In present study, four lab-scale biological annular reactors (BAR) were constructed to investigate the transmission of ARGs exposed to a certain amount of antibiotic (sulfamethoxazole) synergistic disinfectants. It was emphasized that pipe wall biofilm was an important way for ARGs to propagate in the pipeline, and the results were quantified by constructing an operational taxonomic unit (OTU) network map. The network analysis results showed the biofilm contribution to waterborne bacteria was finally estimated to be 51.45% and 34.27% in polyethylen (PE) pipe and ductile iron (DI) pipe, respectively. The proportion of vertical gene transfer (VGT) in biofilm was higher than that in water, and the occurrence of this situation had little relationship with the selection of pipe type. Overall, this study revealed how biofilm promoted the transmission of resistome in bulk water, which can provide insights into assessing biofilm-associated risks and optimizing pipe material selection for biofilm control in DWDS.
由于抗生素的大量使用，饮用水分配系统（DWDS）已成为抗生素耐药性的热点之一。然而，对于生物膜在 DWDS 中传播耐药性方面的作用知之甚少。在本研究中，构建了四个实验室规模的生物环形反应器（BAR），以研究暴露于一定量抗生素（磺胺甲恶唑）协同消毒剂的 ARGs 的传播情况。强调了管壁生物膜是 ARGs 在管道中传播的重要途径，并通过构建操作分类单元（OTU）网络图对结果进行了量化。网络分析结果表明，生物膜对聚乙烯（PE）管和球墨铸铁（DI）管中水生细菌的贡献最终分别估计为 51.45%和 34.27%。生物膜中的垂直基因转移（VGT）比例高于水中的比例，这种情况的发生与管道类型的选择几乎没有关系。总的来说，这项研究揭示了生物膜如何促进耐药组在总水中的传播，这可为评估生物膜相关风险以及优化 DWDS 中生物膜控制的管道材料选择提供见解。
