Programa de Pós Graduação em Ciência Animal (PPGCA), CAV/UDESC, Lages, Santa Catarina, Brazil.
Programa Multicentrico de Pós Graduação em Bioquimica e Biologia Molecular (PMBQBM), CAV/UDESC, Lages, Santa Catarina, Brazil.
J Equine Vet Sci. 2023 Jul;126:104300. doi: 10.1016/j.jevs.2023.104300. Epub 2023 Mar 31.
Campeiro horse is a breed locally adapted to the Santa Catarina plateau region and its main characteristic is the gait, it is known as "Marchador das Araucárias." It is a breed considered in danger of extinction, being fundamental the search for the preservation of this important genetic resource. Surra, caused by the protozoan Trypanosoma evansi, is among the diseases that affect horses. However, there are no data on the prevalence of infection in Campeiro horses. This study aimed to determine the prevalence of T. evansi in Campeiro horses, correlate hematology and serum biochemistry, and identify possible risk factors. Blood samples were collected by venipuncture of 214 Campeiro horses, 50 males and 164 females, aged between 3 months and 27 years, from 16 properties located in the States of Santa Catarina, Rio Grande do Sul, and Paraná. An epidemiological questionnaire was carried out with the owners to analyze the associated risk factors. The blood samples were submitted to polymerase chain reaction, immunofluorescence antibody test, complete blood count, and serum biochemistry. The prevalence was 14% of positive animals by polymerase chain reaction and 59% by immunofluorescence antibody test . There was an increase in hematocrit, and in the number of basophils, a decrease in plasmatic fibrinogen, and in the enzymatic activity of alanine aminotransferase, aspartate aminotransferase, and urea, and an increase in creatine phosphokinase and creatinine in positive animals, which is possibly unrelated to the infection. The data obtained through the epidemiological questionnaires showed no difference. Therefore, T. evansi is present in the South of Brazil, with a high prevalence in Campeiro horses.
坎皮洛马是一种适应圣卡塔琳娜高原地区的本地品种，其主要特征是步态，被称为“阿劳卡里亚斯的三月马”。它是一种被认为处于灭绝危险中的品种，因此寻找保护这一重要遗传资源至关重要。由原生动物伊氏锥虫引起的苏拉病是影响马的疾病之一。然而，关于坎皮洛马感染的流行率尚无数据。本研究旨在确定坎皮洛马感染伊氏锥虫的流行率，对血液学和血清生化进行相关性分析，并确定可能的危险因素。从位于圣卡塔琳娜州、南里奥格兰德州和巴拉那州的 16 个农场的 214 匹坎皮洛马（50 匹公马和 164 匹母马）中采集静脉血样，这些马的年龄在 3 个月至 27 岁之间。对马主进行了一项流行病学调查问卷，以分析相关的危险因素。对血样进行聚合酶链反应、免疫荧光抗体试验、全血细胞计数和血清生化检测。聚合酶链反应阳性动物的流行率为 14%，免疫荧光抗体试验阳性动物的流行率为 59%。阳性动物的红细胞压积和嗜碱性粒细胞数量增加，血浆纤维蛋白原减少，丙氨酸氨基转移酶、天冬氨酸氨基转移酶和尿素的酶活性降低，肌酸磷酸激酶和肌酐升高，这可能与感染无关。通过流行病学调查问卷获得的数据没有差异。因此，伊氏锥虫存在于巴西南部，坎皮洛马的感染率很高。
