Larsson O, Dafgård E, Engström W, Zetterberg A
J Cell Physiol. 1986 May;127(2):267-73. doi: 10.1002/jcp.1041270212.
Proliferating nonconfluent 3T3 cells become committed to proceed through the cell cycle or to enter G0 during the first post-mitotic part of G1 (G1pm). The decision to proceed through G1pm is dependent on the presence of serum growth factors in the culture medium. Cells that have passed this particular growth-factor-dependent cell cycle stage are independent of serum growth factors and undergo mitosis on schedule. We report here that G1ps, S, and G2 cells cease to increase in size when serum is withdrawn. As a result the mitotic cell size after 8 hours serum starvation is reduced to approximately 60% of the normal mitotic cell. This reduced growth in cell size is due to a rapid decrease in protein synthesis and some increase in protein degradation. This dissociation between growth in size and cell-cycle progression within a single cell cycle provides a new approach to study the two processes separately.
增殖的未汇合3T3细胞在G1期的第一个有丝分裂后阶段（G1pm）决定继续进行细胞周期或进入G0期。决定是否继续进行G1pm取决于培养基中血清生长因子的存在。已经通过这个特定的依赖生长因子的细胞周期阶段的细胞不再依赖血清生长因子，并按计划进行有丝分裂。我们在此报告，当血清被去除时，G1ps、S期和G2期细胞的大小不再增加。因此，血清饥饿8小时后的有丝分裂细胞大小减少到正常有丝分裂细胞的约60%。细胞大小的这种减少是由于蛋白质合成的快速下降和蛋白质降解的一些增加。在单个细胞周期内，细胞大小的增长与细胞周期进程之间的这种分离为分别研究这两个过程提供了一种新方法。
