基于 ZnO/CdSe 的光电化学免疫传感用聚丙烯酸/聚乙二醇杂化抗污界面用于 NSE 的检测
Polyacrylic acid/polyethylene glycol hybrid antifouling interface for photoelectrochemical immunosensing of NSE based on ZnO/CdSe.
机构信息
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People's Republic of China.
出版信息
Anal Chim Acta. 2023 May 8;1254:341085. doi: 10.1016/j.aca.2023.341085. Epub 2023 Mar 11.
In this paper, a novel photoelectrochemical (PEC) immunosensor based on ZnO/CdSe semiconductor composite material was constructed to detect neuron-specific enolase (NSE) in a super-sensitive and quantitative way. The antifouling interface composed of polyacrylic acid (PAA) and polyethylene glycol (PEG) can prevent non-specific proteins from adhering to the electrode surface. As an electron donor, ascorbic acid (AA) can increase the photocurrent's stability and intensity by clearing away photogenerated holes. Because of the specific recognition between antigen and antibody, the quantitative detection of NSE can be achieved. The PEC antifouling immunosensor based on ZnO/CdSe has a wide linear range (0.10 pg mL-100 ng mL) and a low detection limit (34 fg mL), which has potential application in the clinical diagnosis of small cell lung cancer.
本研究构建了一种基于 ZnO/CdSe 半导体复合材料的新型光电化学（PEC）免疫传感器，以超灵敏和定量的方式检测神经元特异性烯醇化酶（NSE）。由聚丙烯酸（PAA）和聚乙二醇（PEG）组成的抗污界面可以防止非特异性蛋白质附着在电极表面。抗坏血酸（AA）作为电子供体，可以通过清除光生空穴来增加光电流的稳定性和强度。由于抗原和抗体之间的特异性识别，可以实现 NSE 的定量检测。基于 ZnO/CdSe 的 PEC 抗污免疫传感器具有较宽的线性范围（0.10 pg mL-100 ng mL）和较低的检测限（34 fg mL），在小细胞肺癌的临床诊断中具有潜在的应用价值。
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