苹果多酚对 HO 诱导的人结肠腺癌细胞 Caco-2 氧化应激损伤的保护作用。

Protective Effect of Apple Polyphenols on HO-Induced Oxidative Stress Damage in Human Colon Adenocarcinoma Caco-2 Cells.

机构信息

Department of Pharmacy, Northwest Women's and Children's Hospital.

Center for Drug Evaluation, National Medical Products Administration.

出版信息

Chem Pharm Bull (Tokyo). 2023;71(4):262-268. doi: 10.1248/cpb.c22-00348.

DOI:10.1248/cpb.c22-00348

PMID:37005250

Abstract

Apple is an important dietary agent for human and apple polyphenols (AP) are the main secondary metabolites of apples. In this study, the protective effects of AP on hydrogen peroxide (HO)-induced oxidative stress damage in human colon adenocarcinoma Caco-2 cells were investigated by cell viability, oxidative stress change as well as cell apoptosis. Pre-adding AP could significantly increase the survival rate of HO-treated Caco-2 cells. Besides, the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) were elevated. While the malondialdehyde (MDA) content which is the major oxidant products of polyunsaturated fatty acids (PUFA) reduced after AP treatment. In addition, AP also suppressed the emergence of DNA fragment and decreased the expression of apoptosis-related protein Caspase-3. These results demonstrated that AP could ameliorate HO-induced oxidative stress damage in Caco-2 cells, which could serve as a reference for further studies of apple natural active products and deep study of the anti-oxidative stress mechanism.

摘要

苹果是人类的重要饮食来源之一，苹果多酚（Apple polyphenols，AP）是苹果的主要次生代谢物。本研究通过细胞活力、氧化应激变化和细胞凋亡来探讨 AP 对人结肠腺癌细胞（Caco-2）过氧化氢（Hydrogen peroxide，HO）诱导的氧化应激损伤的保护作用。预先添加 AP 可显著提高 HO 处理的 Caco-2 细胞的存活率。此外，抗氧化酶超氧化物歧化酶（Superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-PX）和过氧化氢酶（Catalase，CAT）的活性也有所提高。而丙二醛（Malondialdehyde，MDA）的含量（多不饱和脂肪酸（Polyunsaturated fatty acids，PUFA）的主要氧化产物）在 AP 处理后降低。此外，AP 还抑制了 DNA 片段的出现，并降低了凋亡相关蛋白 Caspase-3 的表达。这些结果表明，AP 可以改善 HO 诱导的 Caco-2 细胞氧化应激损伤，可为进一步研究苹果天然活性产物和深入研究抗氧化应激机制提供参考。