Division of Cellular and Molecular Toxicology, Center for Biological Safety and Research, National Institute of Health Sciences.
Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University.
J Toxicol Sci. 2023;48(4):203-210. doi: 10.2131/jts.48.203.
Acetamiprid (ACE), a neonicotinoid chemical, is widely used as a pesticide due to its rapid insecticidal activity. Although neonicotinoids exert very low toxicity in mammals, the effects of early exposure to neonicotinoids on the adult central nervous system are poorly understood. This study investigated the effects of ACE exposure in early life on brain function in adult mice. We exposed male C57BL/6N mice to ACE (10 mg/kg) orally when they were two (postnatal lactation) or 11 weeks old (adult). We examined the effects of ACE on the central nervous system using the mouse behavioral test battery, consisting of the open field test, light/dark transition test, elevated plus-maze test, contextual/cued fear conditioning test, and pre-pulse inhibition test at 12-13 weeks old. In the mouse behavioral test battery, learning memory abnormalities were detected in the mature treatment group. In addition, learning memory and emotional abnormalities were detected in the postnatal lactation treatment group. These results suggest that the behavioral effects of postnatal lactation treatment with ACE were qualitatively different from the behavioral abnormalities in the mature treatment group.
乙酰甲胺磷（ACE）是一种新烟碱类化学物质，由于其具有快速的杀虫活性而被广泛用作农药。尽管新烟碱类物质在哺乳动物中毒性很低，但早期接触新烟碱类物质对成年中枢神经系统的影响还知之甚少。本研究旨在探讨早期生活中 ACE 暴露对成年小鼠大脑功能的影响。我们通过口服方式将 ACE（10mg/kg）暴露于 2 周龄（哺乳期后）或 11 周龄（成年期）的雄性 C57BL/6N 小鼠中。我们使用包括旷场试验、明暗交替试验、高架十字迷宫试验、情境/线索恐惧条件试验和预脉冲抑制试验在内的小鼠行为测试组合，在 12-13 周龄时检测 ACE 对中枢神经系统的影响。在小鼠行为测试组合中，成熟处理组出现了学习记忆异常。此外，哺乳期后处理组还出现了学习记忆和情绪异常。这些结果表明，哺乳期后 ACE 处理的行为效应与成熟处理组的行为异常在性质上有所不同。
