Airaudo C B, Gayte-Sorbier A, Momburg R, Laurent P
J Chromatogr. 1986 Feb 28;354:341-54. doi: 10.1016/s0021-9673(01)87035-7.
A thin-layer chromatographic study of 2-mercaptobenzothiazole, 2-(methylthio)benzothiazole, 2-(2-hydroxy-ethylthio)benzothiazole, 2-hydroxybenzothiazole, zinc 2-mercaptobenzothiazolate, mercaptobenzothiazole disulfide, and beta-sitosterol with three developing solvents and eleven spray reagents is reported. A simple method is described which highlights the leaching of 2-mercaptobenzothiazole and 2-(2-hydroxy-ethylthio)benzothiazole from the rubber plunger-seals of disposable syringes: contact of rubber with bidistilled water, extraction of this liquid with chloroform, chromatography on silica gel with the solvents previously studied and spraying with N-chloro-2,6-dichloro-p-benzoquinone monoimine. The method may also be used to discriminate those syringes sterilized with ethylene oxide, the only ones able to leach 2-(2-hydroxy-ethylthio)benzothiazole, from those sterilized by irradiation. Owing to the beta-sitosterol traces leached, it may also be used to investigate whether the plunger-seals are made of natural rubber. The toxic effects of the compounds leached are briefly reported.
报道了用三种展开溶剂和十一种喷雾试剂对2-巯基苯并噻唑、2-（甲硫基）苯并噻唑、2-（2-羟乙基硫基）苯并噻唑、2-羟基苯并噻唑、2-巯基苯并噻唑锌、二硫化巯基苯并噻唑和β-谷甾醇进行的薄层色谱研究。描述了一种简单的方法，该方法突出了一次性注射器橡胶柱塞密封件中2-巯基苯并噻唑和2-（2-羟乙基硫基）苯并噻唑的浸出情况：橡胶与双蒸水接触，用氯仿萃取该液体，用先前研究的溶剂在硅胶上进行色谱分析，并用N-氯-2,6-二氯对苯醌单亚胺喷雾。该方法还可用于区分用环氧乙烷灭菌的注射器（唯一能浸出2-（2-羟乙基硫基）苯并噻唑的注射器）和用辐射灭菌的注射器。由于浸出了痕量的β-谷甾醇，它还可用于研究柱塞密封件是否由天然橡胶制成。简要报道了浸出化合物的毒性作用。
