van der Poll J M, Vink M, Schrijver J, Odink J
J Chromatogr. 1986 Mar 7;375(2):213-23. doi: 10.1016/s0378-4347(00)83714-7.
A gas chromatographic method with alkali flame ionization detection is described for the determination of urinary total (free and conjugated) anthranilic acid (AA) and 3-hydroxyanthranilic acid (HAA) as their pentafluorobenzyl esters. Prior to analysis, urine was hydrolysed using hydrochloric acid in a boiling water bath. The highest AA and HAA yields were obtained with 4 M hydrochloric acid and a hydrolysis time of 4 h. The coefficients of variation of the between-run analyses of AA and HAA at the endogenous level were 7.2 and 5.8%, respectively. The average recovery for both substances was 84%. The method described has been used to study the excretion of AA and HAA in the urine of healthy males and females before and after an oral load of tryptophan. Furthermore, the influence of oral contraceptives has been investigated. Results indicate that for both sexes the excretion of AA in the urine was higher than that of HAA, except after tryptophan loading. The excretion of AA by women was higher than by men. For HAA, the results of both sexes were comparable. Furthermore, for neither of the sexes was a diurnal variation of AA or HAA observed. After tryptophan loading, the formation of HAA was increased by more than that of AA. Results obtained for women on oral contraceptives indicate a hormonal-induced inhibition of AA formation.
描述了一种采用碱火焰离子化检测的气相色谱法，用于测定尿中总（游离和结合）邻氨基苯甲酸（AA）和3 - 羟基邻氨基苯甲酸（HAA），它们以五氟苄酯形式存在。分析前，尿液在沸水浴中用盐酸水解。使用4 M盐酸和4小时水解时间可获得最高的AA和HAA产率。内源性水平下AA和HAA批间分析的变异系数分别为7.2%和5.8%。两种物质的平均回收率为84%。所述方法已用于研究健康男性和女性口服色氨酸负荷前后尿液中AA和HAA的排泄情况。此外，还研究了口服避孕药的影响。结果表明，除色氨酸负荷后外，男女尿液中AA的排泄均高于HAA。女性AA的排泄高于男性。对于HAA，男女结果相当。此外，未观察到男女AA或HAA的日变化。色氨酸负荷后，HAA的生成增加幅度大于AA。口服避孕药的女性所得结果表明激素诱导了AA生成的抑制。
