用于检测实验性表皮葡萄球菌心内膜炎中表皮葡萄球菌抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection of Staphylococcus epidermidis antibody in experimental S. epidermidis endocarditis.
作者信息
Espersen F, Wheat L J, Bemis A T, White A
出版信息
J Clin Microbiol. 1986 Feb;23(2):339-42. doi: 10.1128/jcm.23.2.339-342.1986.
The immune response against Staphylococcus epidermidis, as determined by an enzyme-linked immunosorbent assay, was evaluated in experimental S. epidermidis infections in rabbits. Antigens from 8 of 10 clinical S. epidermidis strains detected significant antibody production in five rabbits immunized with different strains of S. epidermidis and in five of six rabbits with experimental endocarditis caused by four different strains. The antigens from two strains detected antibody production in all rabbits, and strain ATCC 14990 discriminated best between positive and negative samples. Consecutive blood samples from rabbits with endocarditis and control rabbits with bacteremia, which were successfully prevented from developing endocarditis by using prophylactic antibiotics, were examined by using an enzyme-linked immunosorbent assay and an ultrasonic extract of strain ATCC 14990 as the antigen. This assay discriminated between rabbits with endocarditis and rabbits with uncomplicated bacteremia. Antibody production was detected as early as 3 days after the onset of infection in rabbits with endocarditis.
通过酶联免疫吸附测定法确定的针对表皮葡萄球菌的免疫反应，在兔实验性表皮葡萄球菌感染中进行了评估。10株临床表皮葡萄球菌菌株中的8株抗原，在5只接种不同表皮葡萄球菌菌株的兔以及6只由4种不同菌株引起实验性心内膜炎的兔中的5只中，检测到显著的抗体产生。2株菌株的抗原在所有兔中均检测到抗体产生，且ATCC 14990菌株在区分阳性和阴性样本方面表现最佳。使用酶联免疫吸附测定法，并以ATCC 14990菌株的超声提取物作为抗原，对患有心内膜炎的兔和通过使用预防性抗生素成功预防心内膜炎发生的菌血症对照兔的连续血样进行了检测。该检测方法区分了患有心内膜炎的兔和患有单纯菌血症的兔。在心内膜炎兔中，感染开始后3天就检测到了抗体产生。
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