用于快速检测钩端螺旋体生物的辐射测量法。

Radiometric method for the rapid detection of Leptospira organisms.

作者信息

Manca N, Verardi R, Colombrita D, Ravizzola G, Savoldi E, Turano A

出版信息

J Clin Microbiol. 1986 Feb;23(2):401-3. doi: 10.1128/jcm.23.2.401-403.1986.

DOI:10.1128/jcm.23.2.401-403.1986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268657/

Abstract

A rapid and sensitive radiometric method for detection of Leptospira interrogans serovar pomona and Leptospira interrogans serovar copenhageni is described. Stuart's medium and Middlebrook TB (12A) medium supplemented with bovine serum albumin, catalase, and casein hydrolysate and labeled with 14C-fatty acids were used. The radioactivity was measured in a BACTEC 460 (Johnston Laboratories). With this system, Leptospira organisms were detected in human blood in 2 to 5 days, a notably shorter time period than that required for the majority of detection techniques.

摘要

本文描述了一种快速、灵敏的放射测量方法，用于检测波摩那钩端螺旋体和哥本哈根钩端螺旋体。使用了添加牛血清白蛋白、过氧化氢酶和酪蛋白水解物并用14C-脂肪酸标记的斯图尔特培养基和Middlebrook TB（12A）培养基。放射性在BACTEC 460（约翰斯顿实验室）中进行测量。使用该系统，在2至5天内可在人血中检测到钩端螺旋体，这一时间段明显短于大多数检测技术所需的时间。

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引用本文的文献

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Leptospirosis.钩端螺旋体病

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本文引用的文献

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NUTRITION OF LEPTOSPIRA POMONA AND GROWTH OF 13 OTHER SEROTYPES: FRACTIONATION OF OLEIC ALBUMIN COMPLEX AND A MEDIUM OF BOVINE ALBUMIN AND POLYSORBATE 80.波摩那钩端螺旋体的营养及其他13个血清型的生长：油酸白蛋白复合物的分级分离以及牛白蛋白与聚山梨酯80培养基

Am J Vet Res. 1965 Jan;26:45-51.

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Studies of technics for the isolation of leptospires. I. The suitability of 1 to 2 day old baby chicks as compared to hamsters for the isolation of various leptospiral serotypes.钩端螺旋体分离技术的研究。I. 与仓鼠相比，1至2日龄雏鸡用于分离各种钩端螺旋体血清型的适用性。

J Infect Dis. 1958 Sep-Oct;103(2):150-6. doi: 10.1093/infdis/103.2.150.

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Early detection of bacterial growth, with carbon-14-labeled glucose.利用碳-14标记的葡萄糖进行细菌生长的早期检测。

Radiology. 1969 Jan;92(1):154-5. doi: 10.1148/92.1.154.

4

Characterization of leptospires according to fatty acid requirements.根据脂肪酸需求对钩端螺旋体进行特性描述。

J Gen Microbiol. 1969 Mar;55(3):399-407. doi: 10.1099/00221287-55-3-399.

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Radiometric estimation of the replication time of bacteria in culture: an objective and precise approach to quantitative microbiology.

J Nucl Med. 1978 Jun;19(6):619-25.

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