Linh Nguyen Dieu, Huyen Nguyen Thi Trang, Dang Nguyen Hai, Piro Benoit, Thi Thu Vu
University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST) 18 Hoang Quoc Viet, Cau Giay Hanoi Vietnam
Université Paris Cité, ITODYS, CNRS UMR 7086, 15 Rue J.-A. de Baïf Paris F-75013 France.
RSC Adv. 2023 Mar 29;13(15):10082-10089. doi: 10.1039/d3ra00793f. eCollection 2023 Mar 27.
The use of non-invasive approaches for monitoring therapy processes in cancer patients at late stages is truly needed. In this work, we aim to develop an electrochemical interface based on polydopamine combined with gold nanoparticles and reduced graphene oxide for impedimetric detection of lung cancer cells. Gold nanoparticles (around 75 nm) were dispersed onto reduced graphene oxide material pre-electrodeposited onto disposable fluorine doped tin oxide electrodes. The coordination between gold and carbonaceous material has somehow improved the mechanical stability of this electrochemical interface. Polydopamine was later introduced onto modified electrodes self-polymerization of dopamine in an alkaline solution. The result has demonstrated the good adhesion and biocompatibility of polydopamine towards A-549 lung cancer cells. The presence of the two conductive materials (gold nanoparticles and reduced graphene oxide) has led to a six-times decrease in charge transfer resistance of polydopamine film. Finally, the as-prepared electrochemical interface was employed for impedimetric detection of A-549 cells. The detection limit was estimated to be only 2 cells per mL. These findings have proved the possibilities to use advanced electrochemical interfaces for point-of-care applications.
晚期癌症患者治疗过程的无创监测方法确实很有必要。在这项工作中，我们旨在开发一种基于聚多巴胺、结合金纳米颗粒和还原氧化石墨烯的电化学界面，用于肺癌细胞的阻抗检测。将金纳米颗粒（约75纳米）分散到预先电沉积在一次性氟掺杂氧化锡电极上的还原氧化石墨烯材料上。金与含碳材料之间的配位在某种程度上提高了这种电化学界面的机械稳定性。随后通过多巴胺在碱性溶液中的自聚合将聚多巴胺引入修饰电极。结果表明聚多巴胺对A-549肺癌细胞具有良好的粘附性和生物相容性。两种导电材料（金纳米颗粒和还原氧化石墨烯）的存在使聚多巴胺膜的电荷转移电阻降低了六倍。最后，将所制备的电化学界面用于A-549细胞的阻抗检测。检测限估计仅为每毫升2个细胞。这些发现证明了使用先进的电化学界面进行即时检测应用的可能性。
