Cai Juan, Shen Huawei, Wang Yonghong, Peng Yang, Tang Sitian, Zhu Yalin, Liu Qian, Li Baiying, Xie Guoming, Feng Wenli
Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
Clinical Laboratory Medicine Center, Chongqing City Hospital of Traditional Chinese Medicine, Chongqing, 400021, PR China.
Biosens Bioelectron. 2021 Mar 15;176:112893. doi: 10.1016/j.bios.2020.112893. Epub 2020 Dec 15.
Accurate detection of circulating tumor cells (CTCs) has a pivotal role in the metastasis monitoring and prognosis of tumor. In this work, an ultrasensitive electrochemical cytosensor was developed based on excellent electrocatalytic materials and a dual recognition strategy. Herein, novel branched PtAuRh trimetallic nanospheres (b-PtAuRh TNS) were synthesized for the first time by a facile one-pot method, which had a huge specific surface area and outstanding catalytic activity. B-PtAuRh TNS linked with aptamers targeting mucin1 (MUC1) were served as signal tags to amplify the signal. As electrode modified material, the nanocomposites of Cabot carbon black (BP2000) and AuNPs were used to improve the electron transfer efficiency of electrode. In addition to using b-PtAuRh TNS labeled anti-MUC1 aptamers as signal probes, anti-EpCAM antibodies were worked as capture probes to achieve dual recognition of target cells. In other words, only cells expressing both MUC1 and EpCAM could produce electrochemical signal. The constructed cytosensor presented a wide linear range (5 - 1 × 10 cells mL) and a low detection limit (1 cell mL). It was worth noting that the proposed cytosensor could detect CTCs in clinical blood samples. To sum up, the developed cytosensor might become a promising detection platform for cancer diagnosis and tumor metastasis.
循环肿瘤细胞（CTCs）的准确检测在肿瘤转移监测和预后评估中具有关键作用。在本研究中，基于优异的电催化材料和双重识别策略，开发了一种超灵敏的电化学细胞传感器。在此，首次通过简便的一锅法合成了新型树枝状PtAuRh三金属纳米球（b-PtAuRh TNS），其具有巨大的比表面积和出色的催化活性。与靶向粘蛋白1（MUC1）的适配体连接在一起的b-PtAuRh TNS用作信号标签以放大信号。作为电极修饰材料，使用卡博特炭黑（BP2000）和金纳米颗粒的纳米复合材料来提高电极的电子转移效率。除了使用b-PtAuRh TNS标记的抗MUC1适配体作为信号探针外，抗EpCAM抗体用作捕获探针以实现对靶细胞的双重识别。也就是说，只有同时表达MUC1和EpCAM的细胞才能产生电化学信号。构建的细胞传感器呈现出宽线性范围（5 - 1×10细胞/mL）和低检测限（1细胞/mL）。值得注意的是，所提出的细胞传感器能够检测临床血液样本中的CTCs。综上所述，所开发的细胞传感器可能成为癌症诊断和肿瘤转移的一个有前景的检测平台。
